The transformation of 22-hydroxy-23,24-bisnorchol-4-en-3-one to 7a-22-dihydroxy-23,24-bisnorchol-4-en3-one by Botryodiploida theobromae, Lasiodiplodia theobromae, and various Botryosphaeria strains is described. Factors affecting the reaction were incubation temperature, sonication of the substrate, and addition of 2,2'-dipyridyl, extra carbohydrate, and Amberlite XAD-7. The enzyme responsible ...

A double column procedure was devised for simultaneous estimation of norepinephrine, normetanephrine, dopamine, 3-methoxytyramine, and 5-hydroxytryptamine in brain tissue. Columns of aluminum oxide were placed on columns of Amberlite CG-50 by use of a specially devised column holder so that the effluent from the upper columns could flow directly into the lower ones. Perchloric acid extracts of ...

ion Ezchunye Resins-De-Acidite (The Permutit Company, New York), washed on a 40 mesh screen with distilled water, was treated several times with aqueous 4 per cent sodium carbonate and repeatedly washed with distilled water, by suspension and decantation, until the supernatant liquid was colorless and had a pH of 8.0 or less. The resin collected on a suction filter contained 70 to 80 per cent w...

Sir: A new antibiotic, 6"'-O-a-n-mannopyranosyl mannosidostreptomycin has been isolated from the culture filtrate of strain MD753-C2, together with streptomycin and mannosidostreptomycin. This strain has been classified as Streptomyces sioyaensis1) which is a new streptomycin-producer. In this communication, the isolation, characterization and structural elucidation of the antibiotic are report...

A new antitumor antibiotic, named auromomycin, was isolated from the culture broth of Streptomyces macromomyceticus, a macromomycin-producing strain. The antibiotic was recovered from the culture filtrate by salting out with ammonium sulfate and further purified by successive application of ion-exchange chromatography on Amberlite IRA-93 (Cl form) and DEAE-Sephadex (OH form), Gel filtration on ...

Wood, N. P. (A. & M. College of Texas, College Station), and D. J. O'Kane. Formate-pyruvate exchange reaction in Streptococcus faecalis. I. Factor requirement for whole cells. J. Bacteriol. 87:97-103. 1964.-A factor present in plant and animal sources was found necessary for the incorporation of formate-C(14) into pyruvate by Streptococcus faecalis 10Cl. Yeast extract produced a response linear...