Background: The compositions of Ferrous sulphate, Agarose and Xylenol orange dye (FAX) and Ferrous sulphate, Gelatin and Xylenol orange dye (FGX) in solution of distilled water and sulphuric acid are two tissue-equivalent gel dosimeters. Ionizing radiation causes oxidation of Fe ion to Fe ions which diffuse through the gel matrix and blur the image of absorbed dose over a period of hours after ...

1. Rat liver and heart major isoenzymes of NADP-isocitrate dehydrogenase have each been purified about 100-fold by a combination of ammonium sulphate fractionation and chromatography on ion-exchange cellulose and their properties compared. 2. The properties were similar in respect of pH, inhibition by Hg(2+) and Michaelis constants for isocitrate and NADP. 3. Some of the properties of the isoen...

Rat growth hormone was extracted from an acetone powder of transplantable mammosomatotropic pituitary tumors using buffered ammonium sulphate. Active material, precipitated by increasing the ammonium sulphate concentration to 1.5 M, was fractionated first by diethylaminoethyb cellulose chroma tography and then by preparative polyacrylamide gel electro phoresis. The yield of material in the pure...

The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-state fermentation using wheat bran as the substrate. The medium components infl...

Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This review discussed different bromelain purification techniques which will assist in determining the effect of processing conditions on the purification efficacy. There are four purification techniques to be discussed, namely; reverse micellar system, aqueous two phase...

A new spectrophotometric method is proposed for the assay of ranitidine hydrochloride (RNH) in bulk drug and in its dosage forms using ceric ammonium sulphate (CAS) and two dyes, malachite (MAG) green and crystal violet (CV) as reagents. The method involves the addition of a known excess of ceric ammonium sulphate to ranitidine hydrochloride in acid medium, followed by the determination of unre...

The aqueous biphasic system (ABS) method using PEG and ammonium sulphate improved the recovery of spores from tropical soils as compared with the sucrose gradient method. The ABS was not affected by low temperatures. The optimal recovery of spores was achieved when 100% PEG and 50% ammonium sulphate concentration and PEG8000 instead of PEG4000 were used. Under these conditions an optimal interf...

Exo-polysaccharides like dextran produced by Weissella sp. have a wide range of applications in the food, pharmaceutical and other industries. This biopolymer and its derivatives like iron dextran, clinical dextran are rapidly emerging as a new and industrially important products. Dextran a polymer of glucose is produced using sucrose rich media with nitrogen source. It also requires efficient ...

1. The mechanism of sulphite and sulphate permeation into rat liver mitochondria was investigated. 2. Extramitochondrial sulphite and sulphate elicit efflux of intramitochondrial phosphate, malate, succinate and malonate. The sulphate-dependent effluxes and the sulphite-dependent efflux of dicarboxylate anions are inhibited by butylmalonate, phenylsuccinate and mersalyl. Inhibition of the phosp...