background: mutations in β -globin gene may result in β-thalassemia major, which is one of the most common genetic dis­or­ders in iran and some other countries. knowing the beta-globin mutation spectrum improves the efficiency of prenatal diagno­sis in the affected fetuses (major β-thalassemia) of heterozygote couples. methods: couples with high hemoglobin a 2 and low mean corpuscular volume we...

Abstract This study included 80 blood specimens. Fifty samples collected from COVID 19 with age ranged between 02-75 years, and 30 specimens healthy as a control sample 91-63 years. The polymorphism of IFN-γ T/A +874 gene, which amplified by using amplification refractory mutation system (ARMS-PCR) was showed high percentage A allele frequency in patients comparison T frequency, the revealed et...

Abstract Objective The NUDT15 variants impact thiopurine dose selection in acute lymphoblastic leukemia patients. ability to rapidly detect is important clinical practice. This study aims develop a simple polymerase chain reaction (PCR) procedure for detecting Vietnamese Materials and Methods Sanger sequencing was used determine from 200 We designed primers optimized the PCR detection of wild-t...

BACKGROUND JAK2 V617F, a somatic point mutation that leads to constitutive JAK2 phosphorylation and kinase activation, has been incorporated into the WHO classification and diagnostic criteria of myeloid neoplasms. Although various approaches such as restriction fragment length polymorphism, amplification refractory mutation system and real-time PCR have been developed for its detection, a gene...

The calreticulin gene has nine exons and most of the frequent mutations screened observed in 9th exon myeloproliferative neoplasm patients, especially essential thrombocythemia primary myelofibrosis. In current study, an uncommon mutation was a 51 y old man with polycythemia vera phenotype. Somatic novel homozygous affect 9 gene. These recent genetic variations were not reported previous studie...

Abstract The ideal plant architecture is a new strategy for super high yield breeding of rice. Tiller angle an important character A reasonable tiller key factor the and achieving high-yield breeding. Molecular design most potential direction crop in future. development accurate efficient functional molecular markers target trait genes crucial TAC1 ( Angle Controlling ) gene primary that regula...

background: thrombophilia is a main predisposition to thrombosis due to a procoagulant state. several point mutations play key roles in blood-clotting disorders, which are grouped under the term thrombophilia. these thrombophilic mutations are methylenetetrahydrofolate reductase (mthfr, c677t, and a1298c), factor v leiden (g1691a), prothrombin gene mutation (factor ii, g20210a), and plasminogen...