Curcumin was converted into its oligosaccharides by cultured Parthenocissus tricuspidata cells-catalyzed glycosylation. In addition to curcumin 4'-O-glucoside (9%) and curcumin 4',4''-O-diglucoside (14%), two curcumin oligosaccharides, i.e., curcumin 4'-O-gentiobioside (16%) and curcumin 4'-O-gentiobiosyl-4''-O-glucoside (3%), were isolated from P. tricuspidata cells treated with curcumin. ____...

Two beta-glycosidases (BG) (Mr 47,000 and Mr 50,000) were purified from Spodoptera frugiperda (Lepidoptera: Noctuidae) midguts. These two polypeptides associate or dissociate depending on the medium ionic strength. The Mr 47,000 BG probably has two active sites. One of the putative active sites (cellobiase site) hydrolyses p-nitrophenyl beta-D-glucoside (NPbetaGlu) (79% of the total activity in...

In black cherry (Prunus serotina Ehrh.) macerates, the cyanogenic diglucoside (R)-amygdalin undergoes stepwise degradation to HCN catalyzed by amygdalin hydrolase (AH), prunasin hydrolase, and (R)-(+)-mandelonitrile lyase (MDL). A near full-length AH cDNA clone (pAH1), whose insert encodes the isozyme AH I, has been isolated and sequenced. AH I exhibits several features characteristic of beta-g...