Effects of chemical and heat pretreatments on the protein, gluten, and alpha-amylase activity, pasting (Peak [P], Final [F] setback [S] viscosity, pasting temperature [PT] and time [T]) and alveogram (Energy [E], maximum inflation [MI], P/L, and elasticity index [EI]) properties of flour from the pretreated bananas and its composite with wheat flour (WF) were examined. The baking (water absorpt...

OBJECTIVE We examined the effects of caffeine and a psychological stressor on salivary alpha-amylase (sAA) in healthy young males (age 18-30 years) who consumed caffeine on a daily basis. METHODS Using a between-subjects, double-blind, placebo-controlled design, 45 participants received either 200 or 400 mg of caffeine (Vivarin) or placebo, rested for 20 min, and then performed 20 min of ment...

The mean amylase level in 42 human bile samples was 154 IU/l and there was no significant difference in the amylase activity of 32 paired serum and bile samples. Estimation of the amylase thermolability of bile showed it to be similar to that of serum. This suggests that the amylase activity in bile may have filtered through the liver from the hepatic circulation rather than refluxed from the p...

In this rapid quantitative assay for pancreatic alpha-amylase (EC 3.2.1.1) in serum, we precipitate salivary amylases by 10-min incubation with monoclonal anti-salivary amylase antibody immobilized on particles of polyvinylidene fluoride. We then centrifuge the serum mixture and measure the pancreatic amylase activity remaining in the supernate by a kinetic method. The assay requires 50 microL ...

background and objectives: auto-induction is usually employed to achieve high cell density and overproduction of proteins with a simple and low-cost operation. the efficiency of heterologous protein expression in escherichia coli is determined by different parameters. interactions between these parameters usually complicate the identification of those that contribute more to the improvement of ...

1 “Grigore T. Popa” University of Medicine and Pharmacy, School of Pharmacy, Iaşi, Romania 2 “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, School of Veterinary Medicine, Iaşi, Romania ABSTRACT. The purpose of the study was to assess the effect of selenium supplementation on serum amylase, lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activiti...

Examinations of stress in coaches have mainly been qualitative and focused on chronic stressors. This exploratory study examined stress responses in coaches during competition, including psychological and physiological indices. Using reversal theory, we examined metamotivational state profiles during competition. Ten male team sport coaches (mean age 39.8 ± 13.12 years) reported levels of subje...

A rapid assay for serum amylase activity has been developed based on the use of a new chromogenic substrate-Cibachron Blue-amylose. The procedure requires 0.1 ml of serum and measures the production of soluble chro. mogen, the formation of which islinearwith enzyme activity. Normal human serum has a mean amylase activity of 118 ± 37 (SD) mg dye/100 ml/15 mm or, in international units, 78 ± 24 (...

The effect of seed coat removal on the synthesis of alpha-amylase isoenzymes in wheat was investigated. The immature wheat endosperm-aleurone (seed coat and embryo detached) produced considerably less alpha-amylase activity than immature whole or de-embryonated wheat kernels, when incubated under identical conditions of 18.5 C and 99% humidity, in the presence or absence of gibberellic acid (GA...