Levansucrase (EC 2.4.1.10) was purified approximately 40-fold from the extracellular culture fluid of Erwinia herbicola NRRL B-1678. The purified enzyme in its native form occurred as an aggregate. Poly(acrylamide) gel electrophoresis in the presence of sodium dodecyl sulfate showed an active monomer having a molecular weight of 48,000. The enzyme had a K m for sucrose of 28mM and a pH optimum ...

Two types of white wheat bread (high- and low-type loaves) were investigated for rope spoilage. Thirty of the 56 breads tested developed rope spoilage within 5 days; the high-type loaves were affected by rope spoilage more than the low-type loaves. Sixty-one Bacillus strains were isolated from ropy breads and were characterized on the basis of their phenotypic and genotypic traits. All of the i...

This study has developed an innovative method for the production of secondary metabolites starting from Cydonia oblonga Mill (quince) pulp callus culture. The qualitative and quantitative content phenolic triterpenic acids quince extract were elucidated by GC–MS, GC, HPLC-DAD-ESI-MSn. was rich 5-O-caffeoylquinic acid (5-CQA), 5-p-coumaroylquinic (5-p-CoQA) maslinic corosolic acid. Quince extrac...

Pure amylase was isolated from pancreata and parotid glands of the baboon, an animal which has a serum amylase level and renal clearance of amylase (C(Am)) similar to man. After bolus injection, both pancreatic and salivary amylase rapidly disappeared from the serum in a monoexponential fashion with a mean serum half-time of approximately 83 min. Only about 24% of the amylase cleared from the s...

Bacterial amylase from Bacillus subtilis and fungal amylase from Aspergillus niger were potential source for industrial exploitation in the field of amylase production. Hence, this study on bacterial amylase and fungal amylase was done to compare the kinetic parameter of both the types of amylases. 30-60% ammonium sulfate precipitation has done which showed 90 % of purification of both the part...

In this study, Biarum bovei extract was used to produce bioactive peptides from wheat gluten protein and the biological functional properties of hydrolysates were determinated. The results showed that has its highest protease activity (7.3 U/mg protein) at 45 °C pH 5. Based on electrophoresis analysis, molecular weight hydrolysate < 10 kDa. F1 fraction had antioxidant in DPPH (65.85 ± 2.64 µmol...

Previous studies have shown that several factors--such as alloxan-induced diabetes, adrenalectomy, or removal of the thyroid-parathyroid gland complex--can influence the flow rate, protein concentration, and protein composition of rat parotid saliva. The present study was undertaken to explore further the influence of glucocorticoids and thyroxine on rat parotid saliva in hormonally intact anim...