A bifunctional alpha-amylase/subtilisin inhibitor (RASI) was purified to electrophoretic homogeneity from rice (Oryza sativa L.) bran. Its molecular mass was 21 kDa by SDS-PAGE and its isoelectric point was 9.05. Purified RASI inhibited subtilisin Carlsberg strongly and inhibited alpha-amylase from germinating rice seeds weakly. It inhibited rice alpha-amylase more than barley alpha-amylase, an...

In an attempt to identify herbal drugs which may become useful in the prevention of diabetes, antioxidant potentials and α-amylase inhibition by the ethanol extracts of two plants belonging to Lamiaceae family, Otostegia persica and Zataria multiflora, and their different fractions were studied. Also, inhibition of α-amylase by Salvia mirzayanii and its fractions was evaluated. All of the sampl...

diabetes mellitus is one of the most common endocrine diseases and its type ii is the major form of diabetes, accounting for 90% of cases worldwide. the inhibition of carbohydrate hydrolyzing enzymes such as ?-amylase can be an important strategy in the control of blood glucose levels in patients with type ii diabetes. in this investigation, three morus species, m. alba, m. alba var. nigra and ...

To evaluate a new method for measuring pancreatic amylase in serum, in which the salivary isoenzyme is inhibited with a specific monoclonal antibody, we determined the activity of pancreatic and salivary amylase in sera from 103 healthy subjects and from 114 hospitalized patients having a wide range of total amylase activities. CVs for the proposed method ranged from 0.8% to 5.1% (within day) a...

OBJECTIVES The present study was aimed to investigate the α-amylase inhibition and antioxidant activities of methanolic extract of Amaranthus caudatus Linn (MeAc). METHODS Methanolic extract of Amaranthus caudatus was screened for α-amylase inhibition activity by CNPG3 method (2-chloro-p-nitrophenyl-α-D-maltotrioside) and antioxidant activity was evaluated by 1,1-diphenyl-2-picryl-hydrazile (...

Diabetes mellitus is one of the most common endocrine diseases and its type II is the major form of diabetes, accounting for 90% of cases worldwide. The inhibition of carbohydrate hydrolyzing enzymes such as ?-amylase can be an important strategy in the control of blood glucose levels in patients with type II diabetes. In this investigation, three Morus species, M. alba, M. alba var. nigra and ...

This study aimed to evaluate in vitro free radical scavenging activity and anti-diabetic properties of extracts from the sprout Borassus fabellifier. Antioxidant was performed using DPPH, ABTS, FRAP, SO, NO inhibition methods. The enzymes (α-amylase α- Glucosidase inhibitory activities were investigated. Moreover, glucose adsorption diffusion also studied confirm potential effect B. fabellifier...

Diabetes Mellitus is a major health care problem in India with prevalence of about 66.8 million and it was expected to reach 80 million by the year 2025. Type II diabetes (T2DM) accounts for the majority of all diagnosed cases of diabetes in the world with its prevalence increasing sharply in recent decade.The inhibition of alpha-amylase an enzyme involved in the digestion of carbohydrates, can...

introduction: tobacco smoke is involved in the pathogenesis of several diseases in different body systems. saliva is the first body fluid that confront inhaled cigarette smoke which is injurious to the oral cavity and is associated with several oral diseases and cancer. the aim of our study was to evaluate the influence of smoking on the activity of alpha-amylase in the saliva of healthy smoker...