Different organic solvents and detergents showed profound effects on the production of streptomycin (SM), amylases and proteases by Streptomyces griseus strains. The maximum SM production (1128 and 780 mg/L) was obtained using Streptomyces NRRL-strain when ethanol (1.0% v/v) and Tween-80 (0.1%v/v) were added to the medium. Production of amylases was also enhanced using the strain with the addit...

Despite the presence of a family of defense proteins, Phaseolus vulgaris can be attacked by bruchid insects resulting in serious damage to stored grains. The two distinct active forms of α-amylase inhibitors, α-AI1 and α-AI2, in P. vulgaris show different specificity toward α-amylases. Zabrotes subfasciatus α-amylase is inhibited by α-AI2 but not by α-AI1. In contrast, porcine α-amylase is inhi...

The dynamics of mesophilic and thermophilic bacterial population was studied in the bulk soil and rhizosphere of the grass Aristida adscensionis L. in a caatinga of the semi-arid Brazilian Northeast. Mesophilic heterotrophic bacteria, spore–forming bacteria and actinomycetes were significantly more abundant than thermophiles, and their counts were 10 10 CFU g dry soil, showing weak fluctuations...

Zabrotes subfasciatus larvae possess three alpha-amylase isoforms as determined by in gel assays following SDS-PAGE. The two minor isoforms present lower electrophoretic mobility than the major form, and seem to occur as a heterodimer. When developed inside Vigna unguiculata (cowpea) seeds, fourth instar larvae have minor quantities of the slow-migrating forms, but when reared on seeds of Phase...

Investigations were made concerning the number and properties of amylase in sugarcane leaves. Enzymes were extracted froin leaf samples which had been frozen, lyophilized, and ground to a fine powder. Techniques of differential solubihty, gel filtiation, and papei electrophoresis were employed to remove amylase from the crude extracts. Solubility studies showed that amylase was moderately preci...

screening of3000 soil samples collected from cities of four different provinces of iran for starch-hydrolysing bacteria revealed that the nature is enriched with streptomyces species capable of producing amylolytic enzymes. among the bacterial isolates, one of the high starch-degrading strains was selected for further microbiological identification and also amylolytic enzyme(s) characterization...

While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting purifying target from cellular system is also required to characterize enzyme. This is, particular, necessary before developing industrial-scale production. In present study, partially purified α-amylase was obtained strains Pseudogymnoascus sp. Anta...