background: the aim of this study was to evaluate the effects of conjugated lino-leic acid (cla) on apoptosis of tachyzoites of t . gondii , rh strain (type i) and the cyst-forming tehran strain (type ii) in vitro. methods: toxoplasma strains were injected into the peritoneal cavity of balb/c mice. the tehran strain forms cysts in the brain of mice. bradyzoites within the cysts are reactivated ...

Background: Adipose-derived stem cells (ADSCs) are a population of pluripotent cells used for tissue engineering purposes. The main purpose of the present study was to transdifferentiate the ADSCs to Schwann-like cells and to determine the intensity of apoptosis in ADSCs during the transdifferentiation process. Methods: ADSCs were isolated from the inguinal adipose tissue of adult rats and the ...

Studies of cellular apoptosis have been significantly impacted since the introduction of flow cytometry-based methods. Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI ...

Abstract Background Circular RNA pyridoxal kinase (circPDXK; hsa_circ_0061893) is newly identified to be aberrantly expressed in ovarian cancer (OVCA); however, its functional role OVCA cells remains expounded. Methods Real-time quantitative polymerase chain reaction, western blotting, and immunohistochemistry quantified protein expression levels. MiRNA binding site prediction tools predicted d...

INTRODUCTION Enterococcus faecalis is commonly detected in persistent apical periodontitis characterized by unimproved periradicular bone resorption. The aim of the present study was to examine the effect of lipoteichoic acid (LTA), a major virulence factor of E. faecalis, on apoptosis of osteoblasts. METHODS Human osteoblast-like MG63 cells were treated with LTA from E. faecalis at a series ...

conclusions in our study, no prominent effect was seen; therefore, in order to have a better perspective of crocin activity against cancerous cell lines, further studies are highly recommended. results the highest concentration of crocin significantly decreased ros production after 48 hours of treatment. however, crocin had no effect on the expression level of hsp (heat shock protein). addition...

Recent advances in molecular medicine have proposed new therapeutic strategies for cancer. One of the molecular research lines for the diagnosis and treatment of cancer is the use of long non-coding RNAs (LncRNAs) which are a class of non-coding RNA molecules longer than 200 base pairs in length that act as the key regulator of gene expression. Different aspects of cellular activities like cell...

Background and objectives: Cancer cell resistance to common chemotherapy agents is on rise. Plants are considered valuable sources of herbal drugs for cancer therapy. The present study was conducted to investigate the in vitro antioxidant, anti-proliferative, and apoptosis-inducing properties of clove (Syzygium aromaticum L.) extract in human gastric carcinoma...

Colorectal cancer (CRC) is the third most common type of worldwide and a leading cause death. According to Malaysian National Cancer Registry Report 2012–2016, colorectal was second in Malaysia after breast cancer. Recent treatments for colon cases have caused side effects recurrence patients. One alternative ways fight by using natural products. Curcumin compound rhizomes Curcuma longa that po...

background: adipose-derived stem cells (adscs) are a population of pluripotent cells used for tissue engineering purposes. the main purpose of the present study was to transdifferentiate the adscs to schwann-like cells and to determine the intensity of apoptosis in adscs during the transdifferentiation process.   methods: adscs were isolated from the inguinal adipose tissue of adult rats and th...