nucleotide was increased, and, assuming that the product was derived from oligonucleotides and deoxyribonucleoside triphosphates, the synthesis amounted to 74% of the deoxyribonucleic acid primer. 4. The nucleotidyltransferase was inhibited and the lag phase extended by 3'-phosphoryl-terminal oligonucleotides from a splenic-deoxyribonuclease digest of deoxyribonucleic acid. 5. The compounds obt...

Exposure of Escherichia coli cells to myxin results in the almost complete in'hibition of new deoxyribonucleic acid (DNA) synthesis, extensive degradation of pre-existing intracellular DNA, and a rapid loss of viability in these cells (9). After exposure to myxin for 30 min (<1% survivors and >25% degradation of DNA), infection of these cells by T4 bacteriophage results in the renewal of DNA sy...

A DNA sequence polymorphism, revealed by digestion of human DNA with the restriction endonuclease Sst-1 and hybridization with an apolipoprotein A-I complementary DNA clone, has been shown to be located in or close to the 3' noncoding region of the apolipoprotein C-III gene. This polymorphism is found in significantly increased prevalence (P < 0.001) in Caucasian hypertriglyceridemic subjects c...

The forensic biologist has traditionally used a large set of markers including blood group antigens and serum proteins to establish links between evidence and comparison samples. These markers are polymorphic and genetically determined. In 1985, a new technique was introduced which opened the door to the forensic use of deoxyribonucleic acid (DNA) technology: its inventor coined the term ‘DNA R...

When bacteriophage SP02 infects a Bacillus subtilis culture during or shortly after transformation, the frequency of transformants among the resulting lysogens is greatly reduced relative to that in the uninfected culture. The effect can occur after the deoxyribonucleic acid has been taken up and covalently attached to recipient deoxyribonucleic acid.

In a study on Feulgen hydrolysis of frozen-dried alcohol-fixed lily anthers, a chromatographic technique was developed to analyze the acid hydrolysate for some of the degradation products of nucleic acid. Hydrolysis was accomplished by 10 per cent perchloric acid at 20 degrees C., and a typical hydrolysis time-Feulgen intensity curve was obtained, with maximum staining occurring at 19 hours. Mi...

microarray data have an important role in identification and classification of the cancer tissues. having a few samples of microarrays in cancer researches is always one of the most concerns which lead to some problems in designing the classifiers. for this matter, preprocessing gene selection techniques should be utilized before classification to remove the noninformative genes from the microa...

Abstract Background Obesity-related oxidation stress plays a key role in obesity complications; however, its relation to thyroid status is an area for further research. The study aimed assess function obese children and oxidative deoxyribonucleic acid (DNA) damage. Results Fifty 40 normal weight were included . Anthropometric measurement, lipid profile, function, anti-thyroglobulin antibody, pe...