Foot and mouth disease (FMD) is a contagious animal disease that causes irreparable damage to the economy of countries, including Iran in which this disease is a native one. Among the ways to combat FMD are vaccination and slaughter. Due to the specific situation of Iran, it is not possible to kill infected animals. Therefore, vaccination is the most important way to fight this disease. S...

Purpose: Among all known human coronaviruses, some viruses (e.g., SARS-CoV-2) cause severe pneumonia or even death. With the regard to its spread and importance of rapid identification/treatment, because pAbs are relatively cheap, able bind more sites on antigens neutralize them, this study was done for production purification anti-SARS-CoV-2 polyclonal antibodies (pAb) in rats. Methods: Viral ...

Protein purification is not a simple task. Yet, overexpression at bacterial systems with recombinant modifications brings further difficulties. Adding tag, an affinity label, and expressing particular domains of the whole protein, especially hydrophobic sections, make challenging process. folding pattern may perturb N- or C-terminal tag this terminal preference lead to poor yield. Codon optimiz...

background helicobacter pylori multiplies and causes infection in human gastric mucosal layer. new approaches have focused on using specific treatments, such as immunotherapy, to limit this infection. urease, as one of the most important virulent and antigenic factors of the bacterium, is a suitable target for this purpose. patients and methods in order to prepare recombinant proteins, the synt...

A monoclonal antibody prepared against TEM-1 beta-lactamase was found to compete with penicillins and cephalosporins for binding to the enzyme. The purified antibody preparation was linked to Sepharose 4B and used for immunoaffinity-chromatography purification of TEM-1 beta-lactamase. Elution with either benzylpenicillin or cloxacillin yielded a highly purified, concentrated and active enzyme p...

A procedure to immunoaffinity purify the human transcription factor IIH (TFIIH) was developed using a monoclonal antibody that recognizes an epitope in ERCC3 (XPB), the largest subunit of TFIIH. The epitope recognized by the monoclonal antibody was mapped to 20 amino acids. A peptide containing the epitope was capable of displacing TFIIH from an immunoaffinity column containing the monoclonal a...

Introduction Improved purification methods are required for protein structure and function studies, necessitated by growing demands from proteomics, drug development, and biotechnology programs. To simplify purification of recombinant proteins, including many with unknown biochemical properties, several genetically engineered affinity tags, or purification tags, are used. Commonly used tags are...

Abstract Background As the most abundant immunoglobulin in blood and common human isotype used for therapeutic monoclonal antibodies, engagement subsequent activation of its Fc receptors by IgGs are crucial antibody function. While generally assumed to be relatively constant within subtypes, recent studies reveal that variable regions can exert distal effects modulating antibody–receptor intera...