Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...

Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viab...

The antigens of the Egypt virus have been detected by means of the fluorescent antibody technique in the neurons of the brain and spinal cord, in both sensory and motor areas, of infected mice. The antigens have also been observed in infected human epidermoid carcinoma cells in culture. In both cases the antigens occurred in the cytoplasm of the cells exclusively. In the early stages of infecti...

In studies reported elsewhere we have demonstrated by the fluorescent antibody technique that serum of patients with disseminated lupus erythematosus contains a globulin factor with a marked affinity for nuclei.7'8 In further investigation of this reaction it was shown that the component of nuclei involved was in the nucleoprotein fraction." During this study a method for titration of this acti...

VALIDATION OF FAO/IAEA/PANAFTOSA ELISA KIT FOR THE DETECTION OF ANTIBODIES AGAINST FOOTAND-MOUTH DISEASE VIRUS IN VENEZUELA. A liquid phase blocking ELISA (LPBE) supplied by FAO/IAEA/PANAFTOSA has been evaluated for the qualitative and quantitative detection of specific antibodies to "O" and "A" serotypes of foot-and-mouth disease (FMD). A total of 240 bovine sera were analyzed. 120 sera from n...

Human brucellosis is a zoonotic disease which is endemic in Saudi Arabia. The aim of this study was to investigate the humoral immune responses and identify the target antigens that persist at different stages in human brucellosis during antibiotic therapy. To do this, an acute case of accidental nosocomial infection was studied experimentally. Blood was collected from the patient at the time o...