Few cases, if any, of death from drinking diethylene glycol have been reported in this country. This substance is, of course,, largely used as an antifreeze fluid in radiators of rmotor-cars, and is sold under a variety of trade names in this country, in America, and probably elsewhere , without any restrictions. There is little or no mention of the toxicity of this substance in British literat...

Very little is known about the mechanism of antifreeze action of antifreeze glycoproteins (AFGPs) present in Antarctic teleost fish. Recent NMR and CD studies assisted with total synthesis of synthetic AFGP variants have provided insight into the structure of short AFGP glycopeptides, though the observations did not yield information on the antifreeze mechanism of action. In this study, we use ...

Antifreeze (glyco)proteins are found in polar fish species and act to slow the rate of growth of ice crystals; a property known as ice recrystallization inhibition. The ability to slow ice growth is of huge technological importance especially in the cryopreservation of donor cells and tissue, but native antifreeze proteins are often not suitable, nor easily available. Therefore, the search for ...

During cold acclimation, antifreeze proteins (AFPs) that are similar to pathogenesis-related proteins accumulate in the apoplast of winter rye (Secale cereale L. cv Musketeer) leaves. AFPs have the ability to modify the growth of ice. To elucidate the role of AFPs in the freezing process, they were assayed and immunolocalized in winter rye leaves, crowns, and roots. Each of the total soluble pr...

It is sometimes desirable to preserve mammalian cells by hypothermia rather than freezing during short term transplantation. Here we found an ability of hypothermic (+4°C) preservation of fish antifreeze protein (AFP) against rat insulinoma cells denoted as RIN-5F. The preservation ability was compared between type I-III AFPs and antifreeze glycoprotein (AFGP), which could be recently mass-prep...

Antifreeze proteins (AFPs) inhibit ice re-crystallization by a mechanism remaining largely elusive. Dynamics of AFPs’ hydration water and its involvement in the antifreeze activity have not been identified conclusively. We herein, simulation theory, examined reorientation dynamics first layer an AFP from spruce budworm, Choristoneura fumiferana, compared with protein cytochrome P450 (CYP). The ...

A Picture on the front page: 400x magnification of a spiny bristle on a winter-collected Rhagium mordax larvae. The scene is captured using only reflected autofluorescence at 580 nm. Larva is 4if of the 22112011 series (See appendix 6). Foreword This report is the product of a master thesis project made by Johannes Lørup Johnsen at Roskilde University, fall of 2011. It is an integrated master t...

It has been argued that for antifreeze proteins (AFPs) to stop ice crystal growth, they must irreversibly bind to the ice surface. Surface-adsorbed AFPs should also prevent ice from melting, but to date this has been demonstrated only in a qualitative manner. Here we present the first quantitative measurements of superheating of ice in AFP solutions. Superheated ice crystals were stable for hou...