نتایج جستجو برای: antisense plasmid

تعداد نتایج: 49871  

Journal: :Cancer research 1996
C De Giovanni L Landuzzi F Frabetti G Nicoletti C Griffoni I Rossi M Mazzotti L Scotto P Nanni P L Lollini

Human rhabdomyosarcoma cells express membrane epidermal growth factor receptor (ECF-R), which could confer responsiveness to EGF and transforming growth factor-alpha (TGF-alpha) of autocrine or paracrine origin. To study the role played by this growth factor circuit in the proliferation and differentiation of myogenic neoplastic cells, human rhabdomyosarcoma EGF-R-expressing cells (RD/18 clone)...

Journal: :Nucleic acids research 1995
T A Hjalt E G Wagner

Naturally occurring antisense RNAs in prokaryotes are generally short, highly structured and untranslated. Stem-loops are always present, and loop regions serve as primary recognition structures in most cases. Single-stranded tails or internal unstructured regions are required for initiation of stable pairing between antisense and target RNA. Most antisense RNAs contain bulged-out nucleotides o...

Journal: :Journal of the National Cancer Institute 2001
J R Kanwar W P Shen R K Kanwar R W Berg G W Krissansen

BACKGROUND Survivin, a member of the inhibitor of apoptosis (IAP) protein family, is detectable in most types of cancer, and its presence is associated with a poor prognosis. We determined the effects of gene-based therapies that inhibit survivin function in a mouse tumor model. METHODS Using five to six mice per treatment group, we injected tumors derived from mouse EL-4 thymic lymphoma cell...

2003
Mark E. Lasbury Xing Tang Pamela J. Durant Chao-Hung Lee

Alveolar macrophages from Pneumocystis carinii-infected hosts are defective in phagocytosis (W. Chen, J. W. Mills, and A. G. Harmsen, Int. J. Exp. Pathol. 73:709–720, 1992; H. Koziel et al., J. Clin. Investig. 102:1332– 1344, 1998). Experiments were performed to determine whether this defect is specific for P. carinii organisms. The results showed that these macrophages were unable to phagocyto...

Journal: :Proteins 2000
K Steinmetzer A Hillisch J Behlke S Brantl

Plasmid pIP501 encoded transcriptional repressor CopR is one of the two regulators of plasmid copy number. It acts as a transcriptional repressor at the essential repR promoter. Furthermore, CopR prevents convergent transcription from the repR and the antisense promoter, thereby indirectly increasing the amount of antisense-RNA, the second regulatory component. CopR binds as a dimer to a nearly...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2001
W G Jiang D Grimshaw J Lane T A Martin R Abounader J Laterra R E Mansel

PURPOSE Hepatocyte growth factor/scatter factor (HGF/SF), via its receptor c-MET, has been implicated to play a pivotal role in breast cancer development and progression. This study examined a transgene-consisting of a combination of U1snRNA, hammerhead ribozyme, and antisense, designed to inhibit c-met expression-and its impact on the migration and in vitro invasion of breast cancer cells. E...

Journal: :Plasmid 2013
Celeste López-Aguilar Gloria del Solar

Antisense RNAII is a replication control element encoded by promiscuous plasmid pMV158. RNAII binds to its complementary sequence in the copG-repB mRNA, thus inhibiting translation of the replication initiator repB gene. In order to initiate the biochemical characterization of the pMV158 antisense RNA-mediated control system, conditions for in vitro transcription by T7RNA polymerase were set up...

Journal: :American journal of physiology. Heart and circulatory physiology 1999
Xiaoping Tang Dagmara Mohuczy Y Clare Zhang Birgitta Kimura Sara M Galli M Ian Phillips

Angiotensinogen (AGT) has been linked to hypertension. Because there are no direct inhibitors of AGT, we have developed antisense (AS) inhibition of AGT mRNA delivered in an adeno-associated virus (AAV)-based plasmid vector. This plasmid, driven by the cytomegalovirus promoter, contains a green fluorescent protein reporter gene and AS cDNA for rat AGT. Transfection of the plasmid into rat hepat...

Journal: :The Journal of eukaryotic microbiology 2001
M E Lasbury X Tang P J Durant C H Lee

Alveolar macrophages from Pneumocystis carinii-infected hosts are defective in phagocytosis (W. Chen, J. W. Mills, and A. G. Harmsen, Int. J. Exp. Pathol. 73:709-720, 1992; H. Koziel et al., J. Clin. Investig. 102:1332-1344, 1998). Experiments were performed to determine whether this defect is specific for P. carinii organisms. The results showed that these macrophages were unable to phagocytos...

Journal: :Hypertension 1999
D Mohuczy C H Gelband M I Phillips

Vascular smooth muscle cells (VSMCs) are the main peripheral target for vasoconstriction and growth-promoting activity of angiotensin II (Ang II), acting through angiotensin type 1 receptors (AT1-R). Current antihypertension treatments include daily reductions in the effects of Ang II. To decrease an effect of Ang II in a prolonged fashion, we have developed an adeno-associated virus (AAV) vect...

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