A protocol for isolating hemolymph from viruliferous aphids has been developed. This method uses warm melted wax to immobilize the aphid. Following removal of a hind leg, the hemolymph can be collected readily. Flushing with RNase-free water allows for collection of sufficient hemolymph for RNA extraction from individual aphids. The extracted RNA was successfully used for detection of barley ye...

In this study, researchers studied the evolutionary effects on parasitoids of symbiont-mediated resistance by Acyrthosiphon pisum to the parasitoid, Aphidis ervi. They were able to take advantage of the parthenogenic life cycle of the aphid, allowing them to effectively rule out host evolution as the factor effecting results. They performed several experiments to determine what evolutionary eff...

Foraging, defense and waste disposal are essential for sustaining social insect colonies. Hence, their nest generally has an open structure, wherein specialized castes called workers and soldiers perform these tasks. However, some social aphids form completely closed galls, wherein hundreds to thousands of insects grow and reproduce for several months in isolation. Why these social aphids are n...

Lupin plants are frequently damaged by various herbivorous invertebrates. Significant among these are slugs and aphids, which sometimes attack the same plants. Relationships between aphids, slugs and food plant are very interesting. Grazing by these pests on young plants can lead to significant yield losses. There is evidence that the alkaloids present in some lupin plants may reduce grazing by...

Aphids, which feed solely on plant phloem sap, have developed symbiotic associations with bacteria that provide themwith the amino acids that are lacking in phloem. Three soybean aphid (Aphis glycinesMat samura) populations were screened for the presence of Buchnera aphidicola and three common species of secondary aphid symbionts (Serratia symbiotica, Hamiltonella defensa, and Regiella insectic...

This study revealed the tritrophic interactions of predatory coccinellids-host aphids-host plants on pome and stone fruit trees herbaceous in orchards Çanakkale Province, Turkey. Field sampling was done during spring summer seasons 2020 2021. Twelve species belonging to eight genera from family Coccinellidae (Coleoptera) were found interacting with eleven host aphids Aphididae (Hemiptera) plant...