The low critical solution temperature (LCST) and minimum salt concentration that cause phase splitting in water short-chain alcohol mixtures were determined experimentally. alcohols, which are completely miscible with (methanol, ethanol, 1-propanol, 2-propanol, tert-butanol) most of the common salts (those Na+, K+, Ca2+, NH4+ cations Cl–, SO42–, CO32–, NO3– anions), studied. Experimentally data...

This study aimed to purify and partially characterize a keratinolytic protease produced by Bacillus sp. P45 through bioconversion of feather meal. Crude extract was purified using sequence an aqueous two-phase system (ATPS) in large volume systems (10, 50, 500 g) increase obtaining enzyme, followed diafiltration (DF) step. Purified characterized terms protein profile analysis SDS-PAGE, optimum ...

BACKGROUND Too many different protein and enzyme purification techniques have been reported, especially, chromatographic techniques. Apart from low recovery, these multi-step methods are complicated, time consuming, high operating cost. So, alternative beneficially methods are still required. Since, the outstanding advantages of aqueous two phase system (ATPS) such as simple, low cost, high rec...

Aqueous two-phase (ATP) systems are useful in the separation of, particularly, biomolecules. These form when an aqueous solution’s concentrations of phase-forming compounds (such as polymers like polyethylene glycol and salts) within envelope. Various physical chemical conditions affect ATP phase formation, such temperature, pH, composition. One set chemicals which has not been characterized te...

This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). was developed for simultaneous cell cultivation and downstream processing BLIS. Results showed that molecular weight PEG, PEG concentration, dextran T50...

Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of polyethylene glycol (PEG)/dextran-based aqueous two-phase system (ATPS) to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glyco...

In this study, a simple aqueous two-phase system (ATPS) was employed for concurrent purification of oyster polysaccharides. The chemical structure and anti-tumor activities of purified oyster polysaccharides (OP-1) were also investigated. Under optimal ATPS conditions, oyster polysaccharides can be partitioned in the bottom phase with 67.02% extraction efficiency. The molecular weight of OP-1 w...

The paper-based immunoassay for point-of-care diagnostics is widely used due to its low cost and portability over traditional lab-based assays. Lateral-flow immunoassay (LFA) is the most well-established paper-based assay since it is rapid and easy to use. However, the disadvantage of LFA is its lack of sensitivity in some cases where a large sample volume is required, limiting its use as a dia...