The ATDC5 cell line exhibits a multistep process of chondrogenic differentiation analogous to that observed during endochondral bone formation. Previous investigators have induced ATDC5 cells to differentiate by exposing them to insulin at high concentrations. We have observed spontaneous differentiation of ATDC5 cells maintained in ascorbic acid-containing alpha-MEM. A comparison of the differ...

25 The ATDC5 cell line exhibits a multistep process of chondrogenic differentiation analogous to 26 that observed during endochondral bone formation. Previous investigators have induced 27 ATDC5 cells to differentiate by exposing them to insulin at high concentrations. We have 28 observed spontaneous differentiation of ATDC5 cells maintained in ascorbic acid-containing 29 αMEM. A comparison of ...

BACKGROUND Concern has been raised that high levels of ascorbic acid consumption may lead to potential adverse effects, such as vitamin B12 deficiency, iron overload, and kidney stones. OBJECTIVE To examine the relation of serum ascorbic acid level, which reflects intake, to serum vitamin B12 level, serum ferritin level, and kidney stones. METHODS We analyzed data collected on a random samp...

Cadmium (Cd) is a testicular toxicant which induces endoplasmic reticulum (ER) stress and germ cell apoptosis in testes. This study investigated the effects of ascorbic acid on Cd-evoked ER stress and germ cell apoptosis in testes. Male mice were intraperitoneally injected with CdCl(2) (2.0 mg/kg). As expected, a single dose of Cd induced testicular germ cell apoptosis. Interestingly, Cd-trigge...

1. The kinetics of the reaction between HCHO and I-ascorbic acid or chemically related substances have been studied. 2. Indirect evidence is produced for the possible structure of the HCHO-1-ascorbic acid complex. / 3. Lugg's method for determination of I-ascorbic acid has been examined from the point of view of the above experiments, and necessary precautions are stressed. 4. Suggestions for o...

Euglena gracilis was found to contain a peroxidase that specifically requires L-ascorbic acid as the natural electron donor in the cytosol. The presence of an oxidation-reduction system metabolizing L-ascorbic acid was demonstrated in Euglena cells. Oxidation of L-ascorbic acid by the peroxidase, and the absence of ascorbic acid oxidase activity, suggests that the system functions to remove H20...

in order to study the effects of ascorbic acid as an important antioxidant reducing salinity damage, an experiment was conducted on two althernantera genotypes (“entire leaf” and “undulated leaf”) at research greenhouse of shahid chamran university in 2013. concentrations of 0, 0.5 and 1 mm of ascorbic acid were sprayed weekly on plants grown under 0, 30, 60, 90 mm nacl. the experiment was carr...

The effect of ascorbic acid on the monophenolase activity of tyrosinase, using tyrosine as substrate, has been studied. Over the ranges of ascorbic acid concentration used, no direct effect on the enzyme is found. However, a shortening of the characteristic induction period of the hydroxylation reaction is observed. The evolution of the reaction is dependent on the concentration of ascorbic aci...

HYPOTHESIS High-dose ascorbic acid (vitamin C) therapy (66 mg/kg per hour) attenuates postburn lipid peroxidation, resuscitation fluid volume requirements, and edema generation in severely burned patients. STUDY DESIGN AND SETTING A prospective, randomized study at a university trauma and critical care center in Japan. SUBJECTS AND METHODS Thirty-seven patients with burns over more than 30%...