Of 15 L-asparaginase preparations tested, 13 inhibited whole human serum complement; the inhibitory effect ranged from 12 to 45%. We have partially characterized the anticomplementary activity in one of the L-asparaginase preparations. The anticomplementary activity was separable from the L-asparaginase activity on Sephadex G-200. Fractions containing L-asparaginase had no effect either on whol...

Three hundred and sixty four (364) isolates of tropical soil fungi were screened for L-asparaginase production by rapid plate method using modified Czapek-Dox agar containing L-asparagine, and either bromo cresol purple or phenol red dye as an indicator. Results of the study revealed that total one hundred and thirty five (135) isolates showed positive reaction for L-asparaginase production as ...

L-Asparaginase was used to treat 40 patients with acute leukaemia or lymphosarcoma. Fifteen with acute lymphoblastic leukaemia either untreated or in relapse after previous therapy were given "Squibb," "Bayer," or "Porton" L-asparaginase. Five of these patients had complete remission of their disease, and four had good partial remission. Eleven patients with acute myeloid leukaemia were treated...

L-asparaginase is a hydrolase that catalyzes the conversion of L-asparagine--an endogenous amino acid necessary for the function of some neoplastic cells, such as lymphoblasts. In most human cells deficiency of L-asparagine can be compensated by alternative synthesis pathway through which L-asparagine is produced from aspartic acid and glutamine by asparagine synthethase. Depletion of L-asparag...

Asparaginases are known to be the cornerstone for treatment of acute lymphoblastic leukemia (ALL) and are used for treatment in all pediatric regimens as well as in the majority of adult treatment protocols. Clinical hypersensitivity reactions against commercially available asparaginase have resulted in failure of asparaginase in treatment of ALL in more than 60% of cases. Thus, it is required ...

this paper describes optimization method that combines the plackett-burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of l-asparaginase by serratia marcescens sb08. four medium factors, from out of 11 medium factors, were screened by plackett-burman design experiments and subsequent optimization process to find out the o...

the objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular l-asparaginase. a total number of 120 halophilic bacteria were isolated from 17 different saline habitats of iran including salt lakes, wetlands, brine springs and deserts. among these, 68 were able to grow in the presence of 1.5 m nacl and 52 demonstrated the ability to gro...

Cultural factors affecting the production of L-asparaginase by Streptomyces tendae isolated from laterite soil samples of Guntur region were investigated on glycerol-asparagine-salts (modified ISP-5) broth. Optimal yields of L-asparaginase were recorded in the culture medium with the initial pH 7.0 incubated at 30 degrees C for 72 h. The strain utilized sucrose (2%) and yeast (2%) extract as ca...

l-Asparaginase is now known to be a potent antineoplastic agent in animals and has given complete remission in some human leukemias. Extensive clinical trials of this enzyme, however, were not possible in the past because of inadequate production of this substance. We have developed practical procedures for producing l-asparaginase in yields of sufficient quantity and purity for more extensive ...

The antileukemic agent asparaginase triggers the amino acid response (AAR) in the liver by activating the eukaryotic initiation factor 2 (eIF2) kinase general control nonderepressible 2 (GCN2). To explore the mechanism by which AAR induction is necessary to mitigate hepatic lipid accumulation and prevent liver dysfunction during continued asparaginase treatment, wild-type and Gcn2 null mice wer...