this study was aimed to evaluate the effect of gnrh injection on semen ejaculate characteristics in awassi rams collected in breeding and non breeding seasons. the study was conducted in late summer to early autumn when breeding activity commences, and in winter during the non breeding season. twelve mature awassi rams were used in this study. animals were randomly allocated into two equal grou...

background and aims: artichoke (cynara scolymus l.) is a plant of the asteraceae family. this plant is used in herbal medicine. this study was conducted to evaluate the effect of artichokeextract on insulin, glucagon, glucose, alkaline phosphatase (alp), alanine amino transferase (alt) and aspartate amino transferase (ast) in streptozotocin-induced diabetic rats. methods: in this experimental s...

background: this study aims to evaluate the efficacy of silybum marianum seeds (smss) on blood biochemical profile of broiler chickens contaminated with aflatoxin b1 (afb1).  methods: combinations of three levels of afb1 (0, 250 and 500 ppb) with three levels of smss (0, 0.5, and 1.0 %) were incorporated into the basal diet (corn and soybean meal). the effect of nine experimental treatments was...

DNA polymerase (pol) lambda is homologous to pol beta and has intrinsic polymerase and terminal transferase activities. However, nothing is known about the amino acid residues involved in these activities. In order to precisely define the nucleotide-binding site of human pol lambda, we have mutagenised two amino acids, Tyr505 and the neighbouring Phe506, which were predicted by structural homol...

Nα-terminal arginylation (Nt-arginylation) of proteins is mediated by the Ate1 arginyltransferase (R-transferase), a component of the Arg/N-end rule pathway. This proteolytic system recognizes proteins containing N-terminal degradation signals called N-degrons, polyubiquitylates these proteins, and thereby causes their degradation by the proteasome. The definitively identified ("canonical") res...

Laboratory liver tests are broadly defined as tests useful in the evaluation and treatment of patients with hepatic dysfunction. The liver carries out metabolism of carbohydrate, protein and fats. Some of the enzymes and the end products of the metabolic pathway which are very sensitive for the abnormality occurred may be considered as biochemical marker of liver dysfunction. Some of the bioche...

Hepatoprotective activity of the ethanolic extract of the shade dried aerial parts of Portulaca quadrifida Linn. was studied in wister rats using the CCl4 induced hepatotoxicity. The activity was evaluated by using biochemical parameters such as serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin and gamma glutamate transpeptidas...

Hepatoprotective activity of the chloroform extract of the shade dried aerial parts of Solanum trilobatum Linn. was studied in wister rats using the CCl4 induced hepatotoxicity. The activity was evaluated by using biochemical parameters such as serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin and gamma glutamate transpeptidase...

The Escherichia coli gene murZ, encoding the enzyme UDP-N-acetylglucosamine enolpyruvyl transferase, has been cloned and sequenced. Identified by screening an E. coli genomic library for clones that conferred phosphomycin resistance, murZ encoded a 419-amino-acid polypeptide and was mapped to 69.3 min on the E. coli chromosome. MurZ protein was purified to near homogeneity and found to have the...

CoA-transferase (succinyl-CoA-3-oxo acid CoA-transferase, EC 2.8.3.5) isolated from sheep kidney was purified to homogeneity. The purified enzyme has a specific activity of approx. 200 units/mg. A mol.wt. of 110000 was obtained by gel filtration on Sephadex G-200, and a lower mol.wt. of 102000 was determined by analytical ultracentrifugation. A sedimentation coefficient of 5.6S was also determi...