We introduce the use of immobilized enzymes in hydrogels for proteomic sample processing in digital microfluidic systems. In this technique, pre-formed cylindrical agarose discs bearing immobilized enzymes were integrated into digital microfluidic devices bearing arrays of electrodes. In some experiments, fluorogenic enzyme substrates were delivered to gel discs which served as integrated micro...

A light-actuated microfluidic device has been successfully fabricated to inject, move, separate, and merge liquid droplets with nano-liter volumes. Light actuation is realized by sandwiching the liquid droplets between two photosensitive surfaces whose wettability can he changed by light. By integrating a photoconductor with an electrowetting electrode, the surface tension at the liquidsolid in...

Screen-printing was used to incorporate electrodes into a channel of a microfluidic device. These enabled, through the electrochemical oxidation of hydrogen peroxide, the determination of glucose which had been added to milk pre-treated by ultrafiltration. Screen-printing is a relatively simple, inexpensive technique and its use to add electrochemical detection to microfluidic devices is a step...