Biotechnology is integral to the application of robust, high through-put detection of species-specific and species or genus-transferred microsatellites, or simple sequence repeat (SSR) markers. These short, tandemly repeated stretches of DNA of variable motifs and lengths are relatively evenly distributed throughout eukaryotic nuclear, chloroplasts, and mitochondrial genomes. Microsatellites ar...

To increase genetic gain for tolerance to drought, we aimed to identify environmentally stable QTL in per se and testcross combination under well-watered (WW) and drought stressed (DS) conditions and evaluate the possible deployment of QTL using marker assisted and/or genomic selection (QTL/GS-MAS). A total of 169 doubled haploid lines derived from the cross between CML495 and LPSC7F64 and 190 ...

The use of resistant cultivars is the most effective strategy for controlling coffee leaf rust caused by fungus Hemileia vastatrix. To assist development such cultivars, amplified fragment-length polymorphism (AFLP) markers linked to two loci resistance races I and II as well pathotype 001 H. vastatrix were converted sequence-characterized region (SCAR) cleaved polymorphic site (CAPS) markers. ...

Agent technology is critical in providing solutions to grid computing, including resource selection. Traditionally, agent deliberation offers a deductive process whose deliberation cost is very high and also difficult to measure. We consider the use of Multi-Agent Systems (MAS) for grid selection. Within this domain timing is an important index. Once the client applies for some service, the MAS...

Molecular markers are useful for the identification of critical genes controlling agricultural traits of interest in crop germplasm and for the use of these genes in crop improvement using marker-assisted selection (MAS). The improvement of blast disease resistance of rice (Oryza sativa L.) varieties is one of the major goals for rice breeders. The blast resistance (R) gene Pi66(t) was previous...

Markers are any trait of an organism that can be identified with confidence and relative ease, followed in a mapping population or they defined as heritable entities associated the economically important under control polygenes. Molecular markers have diverse applications fruit crop improvement, particularly areas genetic diversity varietal identification studies, disease diagnostics, hybrid de...

Cultivated maize (Zea mays L.) is the oldest and one of most important crop species in world. Changing climatic conditions recent years, warm weather, expansion acreage intensification cultivation have resulted an increase threat posed by diseases caused by, among others, Fusarium fungi. Breeding success all plant determined access to starting materials with possible high genetic diversity also...

linked and/or gene-based molecular markers have been used widely in marker-assisted selection (mas) to differentiate resistant and susceptible genotypes. resistance to meloidogyne spp. in beta vulgaris l. is mediated by a single dominant gene (r6m-1). using allele-specific primers (asps), an snp marker harboring a single nucleotide polymorphism (a/g), linked to the resistance gene was developed...

Perennial crops are vital contributors to global food production and nutrition. However, the breeding of new perennial crops is an expensive and time-consuming process due to the large size and lengthy juvenile phase of many species. Genomics provides a valuable tool for improving the efficiency of breeding by allowing progeny possessing a trait of interest to be selected at the seed or seedlin...