INTRODUCTION: Bone remodeling, a dynamic process involving bone resorption followed by bone formation, takes place throughout life to maintain bone homeostasis. Osteoporosis pseudoglioma (OPPG) syndrome and high bone mass (HBM) syndrome are both mapped to the locus of Lrp5, a receptor for Wnt ligands in activation of β-catenin signaling (1). OPPG patients harbor inactivating mutations in the Lr...

Follicular development occurs in wave like patterns in monotocous species such as cattle and humans and is regulated by a complex interaction of gonadotropins with local intrafollicular regulatory molecules. To further elucidate potential mechanisms controlling dominant follicle selection, granulosa cell RNA harvested from F1 (largest) and F2 (second largest) follicles isolated at predeviation ...

Background: In the absence of Wnt stimulation, the transcriptional cofactor b-catenin is destabilized via phosphorylation by protein kinase GSK3b in complex with Axin family members. In the ‘‘canonical’’ Wnt signaling pathway, Disheveled (Dvl) is required to functionally inhibit the activity of the GSK3b/Axin complex and thereby stabilize b-catenin. Yet, the mechanisms that underlie Wnt regulat...

Wnt/β-catenin signalling regulates cell proliferation by modulating the cell cycle and is negatively regulated by conductin/axin2/axil. We show that conductin levels peak at G2/M followed by a rapid decline during return to G1. In line with this, Wnt/β-catenin target genes are low at G2/M and high at G1/S, and β-catenin phosphorylation oscillates during the cell cycle in a conductin-dependent m...