Cyclopropane fatty acids constitute 25% of the phospholipid acyl groups in cysts of Azotobacter vinelandii. These are lost by dilution during germination when the synthesis of the fatty acids characteristic of vegetative cell phospholipids commences.

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Azotobacter synthesizes an extensive internal membranous nework when grown with air (N(2)), i.e., under conditions when these bacteria fix nitrogen. Very slight quantities of internal membrane, concentrated mainly about the cell periphery, are formed when Azotobacter grows with fixed nitrogen, i.e., ammonia and amino acids. Compared to cells growing with ammonia, cells utilizing atmospheric nit...

Ornithine decarboxylase (ODC), cyclin D1 (CCND1) and antizyme inhibitor (AZI) promote cell growth. ODC and CCND1 can be degraded through antizyme (AZ)-mediated 26S proteasomal degradation. This paper describes a mechanistic study of the molecular interactions between AZ and its interacting proteins. The dissociation constant (Kd) of the binary AZ-CCND1 complex and the respective binding sites o...

The present investigation “The effect of biofertilizers on growth, yield and quality Cape Gooseberry (Physalis peruviana L.) in Prayagraj agroclimatic conditions” was undertaken at Central Research Field, Department Horticulture, Naini Agricultural Institute, Sam Higginbottom University Agriculture, Technology & Sciences, (UP) during 2022-2023. main objective the experiment to find out vari...

Strains of aerobic, microaerobic, nonsymbiotic, and symbiotic dinitrogen-fixing bacteria were screened for the presence of alternative nitrogenase (N2ase) genes by DNA hybridization between genomic DNA and DNA encoding structural genes for components 1 of three different enzymes. A nifDK gene probe was used as a control to test for the presence of the commonly occurring Mo-Fe N2ase, a vnfDGK ge...

Temperature affects the expression of the three different nitrogenases in Azotobacter vinelandii. Molybdenum repressed the vnfH and anfH operons relatively more at 30 degrees C than at 20 degrees C; at 14 degrees C molybdenum did not repress these genes at all. Similarly, V repressed the anf operon at 30 degrees C but not at 20 or 14 degrees C. Mo was poorly transported into cells grown at the ...

Pleomorphism in cultures of Azotobacter vinelandii was induced by addition of Difco peptone to the growth medium. Under the conditions of the study, 5% peptone gave rise to transient forms described as "fungoid cells" which were osmotically fragile. After some 48 hr of culture, they became osmotically stable and resumed a more typical morphology. It was shown that the pleomorphism-inducing prin...

The site or sites that protect nitrogenase from O(2) inactivation in vivo are sensitive to sodium azide or 2,4-dinitrophenol. Both components of nitrogenase can be synthesized when oxidative phosphorylation is disrupted.

To assess the immunological cross-reactivity of the monobactam antibiotic aztreonam (AZ), rabbits were immunized with protein conjugates of benzylpenicillin, cephalothin (CEPH), and AZ. The resulting antibenzylpenicilloyl (BPO) and anti-CEPH rabbit antibodies showed negligible cross-reactivity with AZ conjugated to human serum albumin (AZ-HSA), whereas anti-AZ showed negligible cross-reactivity...