Decolorization of azo dye Red 3BN by two bacterial species Bacillus cereus and B. megaterium has been analyzed using mineral effluent, consisting of known concentration of the dye in ZZ medium. Physico chemical parameters like carbon source, nitrogen source, temperature, pH and inoculum volume are optimized for the decolorization process by changing one parameter at a time. Optimal condition fo...

Mung bean nuclease treatment of 16S-23S ribosomal DNA intergenic transcribed spacers (ITS) amplified from several strains of the six species of the Bacillus cereus group showed that B. anthracis Davis TE702 and B. mycoides G2 have other intermediate fragments in addition to the 220- and 550-bp homoduplex fragments typical of the B. cereus group. Long and intermediate homoduplex ITS fragments fr...

The first guidance for applicants on how to assess toxigenic potential of species of the genus Bacillus was developed by the then Scientific Committee on Animal Nutrition (SCAN) and published in 2000 with the title Opinion on the Safety of use of Bacillus species in Animal Nutrition (EC, 2000). The SCAN guidance took as its basis the then existing knowledge on the structure and biogenesis of to...

Bacillus cereus group is widespread in nature and foods. Several members of this group are recognized as causing food spoilage and/or health issues. This study was designed to determine the prevalence and genetic diversity of the B. cereus group strains isolated in Tunisia from different foods (cereals, spices, cooked food, fresh-cut vegetables, raw and cooked poultry meats, seafood, canned, pa...

Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins having characteristics different from those of streptolysin O.

Bacillus cereus infections The importance of Bacillus organisms, particularly B. cereus, in local infections has been largely overlooked because they are commonly encountered as contaminants in specimens or cultures. Turnbull et al. (J Clin Pathol 1979 ;32 :289-93) reported severe infections with B. cereus and prompted me to review the recent isolates in this laboratory. In the nine months, Nov...

AIMS Our aim was to assess the diversity of the nutrient germination response of Bacillus cereus spores. METHODS AND RESULTS B. cereus spore germination was monitored by decrease in optical density using a Bioscreen C analyser in response to the major germinant substances inosine and l-alanine. Spores of a set of 12 strains taken to illustrate the diversity of the B. cereus group showed rangi...

Plant-parasitic nematodes cause serious crop losses worldwidely. This study intended to discover the antagonistic mechanism of Bacillus cereus strain S2 against Meloidogyne incognita. Treatment with B. cereus strain S2 resulted in a mortality of 77.89% to Caenorhabditis elegans (a model organism) and 90.96% to M. incognita. In pot experiment, control efficiency of B. cereus S2 culture or supern...

In the present study we characterized 47 food-borne isolates of Bacillus cereus using multilocus sequence typing (MLST). Newly determined sequences were combined with sequences available in public data banks in order to produce the largest data set possible. Phylogenetic analysis was performed on a total of 296 strains for which MLST sequence information is available, and three main lineages--I...

The facultative anaerobe, Bacillus cereus, causes diarrheal diseases in humans. Its ability to deal with oxygen availability is recognized to be critical for pathogenesis. The B. cereus genome comprises a gene encoding a protein with high similarities to the redox regulator, Rex, which is a central regulator of anaerobic metabolism in Bacillus subtilis and other Gram-positive bacteria. Here, we...