In experiments using cultured cells, LS2616 has been shown to decrease growth of primary tumors and pulmonary metastasis of murine melanoma. In the current study, we examine the efficacy of LS2616 for the prophylactic and therapeutic treatment of metastases from ocular and flank inoculations of the highly aggressive in vivo derived B16F10 melanoma in C57BL/6J mice. Experimental animals were tre...

Epidemiological data suggest there is a female sex advantage in melanoma skin cancer. Female sex hormones have been attributed to this protection. There has been no experimental evidence to link female sex hormones directly with melanoma protection until our recently published work on mouse melanoma (B16F10) cells. Our recently published work showed that progesterone significantly inhibited mou...

In the previous study, we generated a monoclonal antibody, 8F11, against NL-17, a high metastatic clone derived from a metastatic variant of murine colon adenocarcinoma 26. 8F11 inhibited platelet aggregation induced by NL-17 and recognized a Mr 44,000 membrane protein as antigen. In the present study, the reactivity of 8F11 to murine B16 melanoma and its metastatic variants was examined, and t...

Purpose We investigated the application of contrast-enhanced ultrasound (CEUS) to quantify vascular perfusion in two animal models of choroidal melanoma. Methods B16F10 and choroidal melanoma cell line (OCM1) melanoma xenografts were implanted into the cyclodialysis cleft of rabbits' right eyes. Fundus photography, indocyanine green angiography (ICGA), B-mode ultrasound, and CEUS were used to...

Both cell-autonomous and non–cell-autonomous factors contribute to tumor growth and metastasis of melanoma. The function of caveolin-1 (Cav1), a multifunctional scaffold protein known to modulate several biologic processes in both normal tissue and cancer, has been recently investigated inmelanoma cancer cells, but its role in the melanoma microenvironment remains largely unexplored. Here, we s...

Over the past few decades, melanoma has shown the fastest growing incidence rate of all cancers. This malignancy is clinically defined by its potential to rapidly metastasize, and advanced metastatic melanomas are highly resistant to existing therapeutic regimens. Here, we report that PPI-2458, a novel, orally active agent of the fumagillin class of irreversible methionine aminopeptidase-2 (Met...

Anti-PD1 therapy revolutionized melanoma treatment, yet low response rates and immune-related adverse events remain a major concern. Caspase-1 inflammasome is autonomously active in known to promote tumor progression. Therefore, we investigated its role immunotherapy resistance melanoma. The pharmacological inhibition of caspase-1 augmented mouse (B16F10 YUMM1.7) anti-PD1 vivo. combination stud...

Abstract Melanoma is an aggressive skin cancer that develops from the malignant transformation of pigment-producing cells, melanocytes. The incidence cutaneous melanoma remarkably high, with estimated number new cases in United States 2022 close to 100,000 patients. Several previous reports pointed out effect complement system progression melanoma, although precise mechanism largely unknown. a ...

Ubiquitous pro-oxidative stressor ultraviolet B radiation (UVB) to human or mouse skin generates platelet-activating factor (PAF) and novel oxidatively modified glycerophosphocholines (Ox-GPCs) with PAF-receptor (PAF-R) agonistic activity. These lipids mediate systemic immunosuppression in a process involving IL-10. The current studies sought to determine the functional significance of UVB-medi...