The immunity induced in mice by vaccination with living attenuated cultures of tubercle bacilli was measured by two criteria. (a) Increase in survival time of the vaccinated animals after infection with a dose of virulent bacilli sufficient to kill all the unvaccinated controls within 10 to 20 days. (b) Difference in the number of living bacilli recovered from the spleen and lungs of vaccinated...

1. A vaccine prepared from partially defatted bovine tubercle bacilli influenced favorably the survival time after inoculation with living virulent bovine tubercle bacilli. 2. Accompanying this increased resistance was a sustained lymphocytosis in the vaccinated animals. 3. The vaccine induced a transitory anemia and leucopenia. 4. A similar preparation of partially defatted human tubercle baci...

Comparisons were made in Lusaka and in London between manual staining and staining in an automatic machine with auramine-phenol of direct smears of sputum and other types of specimen for acid-fast bacilli. No evidence was obtained of carry-over of acid-fast bacilli from positive to negative smears during machine staining. There was improved contrast between bacilli and the background in smears ...