The protonophore-mediated collapse of the large delta pH that acidophiles maintain across their cytoplasmic membranes was augmented by the presence of Cl-, and Cl- influx into the cells occurred evidently in response to the protonophore-induced increase in the inside-positive membrane potential (+ delta psi). In respiring cells, the addition of Cl- but not SO4(2-) salts caused a rapid and preci...

Probiotics are microorganisms which when administered in adequate amounts confer health benefits to the host. A leading pharmaceutical company producing Bacillus coagulans as a probiotic was facing the problem of recurring phage attacks. Two mutants viz. B. co PIII and B. co MIII that were isolated as phage resistant mutants after UV irradiation and MMS treatment of phage sensitive B. coagulans...

Effects of high temperature pretreatment and inoculation Bacillus coagulans were determined relative to the physicochemical properties bacterial community aerobic composting chicken manure. Chicken manure was pretreated with for 0 h (CJ), 0.5 (T-0.5h), 1.0 (T-1.0h), 1.5 (T-1.5h), 2.0 (T-2.0h) then inoculated B. coagulans. without included as control (CK). The results showed that in CJ, T-0.5h, ...

The experiment was carried out to examine the organic acids production of rice straw fermented with some types of microorganisms at different temperatures. The experiment was designed as Split Plot-Completely Randomized Design. The main plot was temperatures treatments (25, 35, 45°C) and the sub plot were microorganisms (Control, Control+Mollases, Lactobacillus fermentum, Bacillus subtilis, Bac...

Bacillus coagulans 2-6 is an excellent producer of optically pure l-lactic acid. However, little is known about the mechanism of synthesis of the highly optically pure l-lactic acid produced by this strain. Three enzymes responsible for lactic acid production-NAD-dependent l-lactate dehydrogenase (l-nLDH; encoded by ldhL), NAD-dependent d-lactate dehydrogenase (d-nLDH; encoded by ldhD), and gly...

This study investigated the feasibility of producing L-lactic acid (LA) from dry corn stalk (DCS) that was pretreated by ensiling an anaerobic microbial community consisting Bacillus coagulans, Lactobacillus fermentum, and Enterococcus durans. After 28 days ensiling, LA acetic content in microsilage 2.04 ± 0.08% 0.38 0.01%, respectively, pH 4.47 0.13. became dominant microbiota during process. ...

A protease-sensitive antibacterial substance produced by Bacillus coagulans I4 strain, isolated from cattle faeces, was classified as a bacteriocin-like inhibitory substance and named coagulin. The inhibitory spectrum included B. coagulans and unrelated bacteria such as Enterococcus, Leuconostoc, Oenococcus, Listeria and Pediococcus. Coagulin was stable at 60 degrees C for 90 min, at a pH rangi...