The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive beta-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment.

The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the outer membrane fraction of E. coli, and virtually no soluble penicillinase was f...

this study investigated the inhibitory effects of bacillus licheniformis on g1 and g2 aflatoxins in culture media and kilka fish meal. two concentrations of aflatoxins (8 and 12 ppb for g1 and g2) and (2 and 4 ml from aflatoxins standard per 50g of fish meal) were used in culture media and kilka fish meal respectively. the doses of bacillus were 3 and 4% for this experiment. changes of aflatoxi...

Novel cellulolytic microorganisms are becoming more important for rapidly growing biofuel industries. This paper reports the draft genome sequences of Bacillus licheniformis strains YNP2-TSU, YNP3-TSU, and YNP5-TSU. These cellulolytic isolates were collected from several hydrothermal features inside Yellowstone National Park.

Bacilli are genetically and physiologically diverse, ranging from innocuous to highly pathogenic. Here, we present annotated genome assemblies for 20 strains belonging to Bacillus anthracis, B. atrophaeus, B. cereus, B. licheniformis, B. macerans, B. megaterium, B. mycoides, and B. subtilis.

The penicillinase antirepressor gene, penJ, of Bacillus licheniformis ATCC 9945a was cloned in Escherichia coli by using pMB9 as a vector plasmid. The penicillinase gene, penP, its repressor gene, penI, and penJ were encoded on the cloned 5.2-kilobase HindIII fragment of the recombinant plasmid pTTE71. The penJ open reading frame was composed of 1,803 bases and 601 amino acid residues (molecula...

Production of Polyglutamate (PGA) biopolymer by immobilized Bacillus licheniformis strain-R was intensively investigated. Preliminary experiments were carried out to address the most suitable immobilization methodology. Entrapment of Bacillus cells in alginate-agar led optimal PGA production (36.75 g/l), with 1.32-and 2.18-fold increase in comparison with alginate-or K-carrageenan-immobilized c...

Keratin are insoluble fibrous proteins found in hair, wool, feather, nail, horns and other epithelial covering which is rich in beta helical coil linked through cysteine bridges. Keratinase (EC 3.4.4.25) belongs to the class hydrolase which are able to hydrolyse insoluble keratins more efficient than other proteases. The bacteria Bacillus licheniformis showing higher keratinase activity was scr...

The reversibility of thermal denaturation and catalytic efficiency of Bacillus licheniformis α-amylase were improved through site-directed mutagenesis. By using multiple sequence alignment and PoPMuSiC algorithm, Ser187 and Asn188, which located within a long loop in Domain B of Bacillus licheniformis α-amylase, were selected for mutation. In addition, Ala269, which is adjacent to Ser187 and As...

Hydrogen is a clean and efficient fuel, considered as a potential and more sustainable energy substitute for fossil fuels. Biological hydrogen production stands out as an eco friendly process carried out under mild operating conditions with renewable resources. In the current work laboratory scale production of hydrogen using phototrophic purple non-sulphur bacteria Rhodobacter sphaeroides, ana...