The structure of rat liver vault ribonucleoprotein particles was examined using several different staining techniques in conjunction with EM and digestion with hydrolytic enzymes. Quantitative scanning transmission EM demonstrates that each vault particle has a total mass of 12.9 +/- 1 MD and contains two centers of mass, suggesting that each vault particle is a dimer. Freeze-etch reveals that ...

We report a novel approach to a new class of bioengineered, monodispersed, self-assembling vault nanoparticles consisting of a protein shell exterior with a lipophilic core interior designed for drug and probe delivery. Recombinant vaults were engineered to contain a small amphipathic α-helix derived from the nonstructural protein 5A of hepatitis C virus, thereby creating within the vault lumen...

Compressive tests on clay tiles used in historical masonry timbrel vaults are hindered by the relatively small thickness of specimens, resulting buckling or confinement problems depending loading direction. This paper presents an experimental campaign and a numerical validation novel testing setup for estimating compressive strength thin vaults. The focuses two different types corresponding to ...

Abstract. This paper presents a part from one broader research project on ribbed vaults. The main goal is to generate parametric objects library for vaults, suitable both HBIM system, structural analysis or Cultural Heritage dissemination. Starting Treatises study we have analyzed different classification system and terminology used vaults components in languages, especially English, French, Sp...

There is a wide range in the reported photon tenth value distance (TVD) in the maze of high-energy linear accelerator vaults. In order to gain insight into the appropriate use of the TVD value during door design, we performed measurements of the photon dose in the maze of four vaults. In addition, our study represents the first to describe a scenario where an inner borated polyethylene (BPE) do...

Human vaults are the largest cytoplasmic ribonucleoprotein and are overexpressed in cancer cells. Vaults reportedly function in the extrusion of xenobiotics from the nuclei of resistant cells, but the interactions of xenobiotics with the vault-associated proteins or non-coding RNAs have never been directly observed. In the present study, we show that vault RNAs (vRNAs), specifically the hvg-1 a...

Neutron and photon dose equivalents at various points along the mazes of the vaults for two 15 MV linear accelerators were measured. The measurements were made with the machines set at various gantry angles with and without the scattering phantom, and with the collimators set at the maximum and the minimum field sizes. Neutron dose equivalent measurements were made for five other accelerator va...