Basil (O. gratissimum L) is a aromatic and medicinal plant widely used in traditional medicine in Morocco. The aim of this work was to study the effect of three plant growth regulators gibberellic acid (GA), indole 3-acetic acid (IAA) and benzylaminopurine (BAP) on the content and composition of essential oils of this plant, especially on the main compound (methyl chavicol) and its isomer (the ...

An efficient regeneration protocol was developed from shoot tip and nodal explants of Simarouba glauca DC, a promising biodiesel plant. Nodal explants appeared to have better regeneration capacity than shoot tip explants (40%) in the tested media. The highest regeneration frequency (90%) and shoot number (7.00 ± 1.00 shoots per explants) were obtained in nodal explants in Murashige and Skoog’s ...

In order to investigate the genetic variation in tissue culture response and to ®nd the cultivars with high regeneration ability for genetic transformation, twenty®ve turf-type tall fescue (Festuca arundinacea Schreb.) cultivars, including many elite ones released recently, were evaluated for their callus induction and plant regeneration responses. Callus induction was initiated from mature see...

The influence of pre-sowing treatments using hydropriming, osmopriming and halopriming in laboratory and/or nursery tests on seedling emergence, seedling weight and plant growth of endive and chicory was examined. Halopriming (KNO3) or growth regulators (gibberelic acid; GA3) improved the rate of germination of endive and chicory and reduced the mean germination time needed. 30 min pre-sowing t...

An efficient in vitro regeneration protocol has been developed from shoot tips of Drosera burmannii Vahl., a carnivorous plant of north-east India. Various plant growth regulators were used to study their efficacy in the induction of multiple shoots and roots. Of the various treatments, the maximum number of shoots (28.8 ± 1.5) and roots (9.7 ± 0.6) was observed in one-fourth strength standard ...

Pigeonpea is an important grain legume, however the genetic transformation of pigeonpea has been very challenging to date. In the present study, we describe a simple protocol for plantlet regeneration via multiple shoot induction from 12-16 day old immature embryos of pigeonpea cultivar ICPL 87 which can be used for efficient transformation. By optimizing the culture medium, we have achieved or...

In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10-15 year old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) suppleme...

Abstract Hairy root cultures are an efficient tool for the biotechnological production of plant metabolites and a convenient experimental model analyzing effect various compounds on metabolism. In contrast to many other types in vitro cultures, hairy roots do not require external supply phytohormones medium. Consequently, growth regulators such as auxins cytokinins rarely used elicitors culture...

The in vitro biocontrol ability of Trichoderma harzianum on the phytopathogen Alternaria alternata improved in the presence of the growth regulators gibberellic acid (GA3), or indolacetic acid (IAA) or benzylaminopurine (BAP) or foliar nutrient at concentrations similar or higher than those used at the field level. These plant hormones decreased the secretion of endopolygalacturonase (endo-PG) ...

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male fl owers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic em...