The enantiomeric separation of three selenoamino acids, D,L-selenomethionine, D,L-selenoethionine and D,L-selenocystine is described. Both sulfated beta-cyclodextrin and vancomycin have been successfully used to separate all enantiomers of the compounds with UV detection. Reproducible separations, in terms of peak area and migration time were obtained using sulfated beta-cyclodextrin with rever...

Submit Manuscript | http://medcraveonline.com Abbreviations: HPMC: Hydroxypropyl Methylcellulose; MC: Methylcellulose; PLGA: Poly (Lactide-co-Glycolide); CAP: Cellulose Acetate Phthalate; LCST: Low Critical Solution Temperature; UCST: Upper Critical Solution Temperature; PPO: Polypropylene Oxide; PEO: Polyethylene Oxide; MTA: Methozolamide; PNIPAAm: Poly (N-Isopropylacrylamide); GC: Guluronic A...

BACKGROUND/AIMS F508delCFTR-, but not wtCFTR-, expressing fibroblasts resemble Niemann Pick type C cells in the massive intracellular accumulation of free cholesterol. The recruitment and activation of F508delCFTR by cholesterol depletion was studied. METHODS Filipin staining, forskolin-stimulated anion efflux and FITC-dextran uptake were studied in control cells and fibroblasts treated with ...

Omeprazole (OME) is a proto type anti-secretary agent. This compound is very unstable, especially in acidic aqueous solutions. A stability indicating HPLC assay has been developed in order to investigate the effect of different factors on the stability of omeprazole. These factors included the pH with the tested values ranging from 6-10 and the temperature by monitoring the drug stability at 25...

beta-Cyclodextrin dimers bearing an oxamido bis(2-benzoic) carboxyl linker (1) or its metal complexes (2 and 3) were newly synthesized, and their inclusion complexation behavior with a series of representative aliphatic oligopeptides, i.e., Leu-Gly, Gly-Leu, Gly-Pro, Glu-Glu, Gly-Gly, Gly-Gly-Gly, and Glu(Cys-Gly), was elucidated by means of UV/vis, circular dichroism, fluorescence, and 2D NMR ...

Enantiomer separation of mandelates and their analogs, which are important intermediates in asymmetric synthetic and pharmaceutical chemistry, was investigated by capillary gas chromatography using different cyclodextrin derivative chiral stationary phases (CD CSPs). The used cyclodextrin derivatives included permethylated beta-CD (PMBCD), permethylated gamma-CD, heptakis(2,6-di-O-butyl-3-O-but...

An on-line coupled capillary isotachophoresis-capillary zone electrophoresis (cITP-CZE) method for the determination of the fumaric acid content in apple juice is presented. A clear separation of fumaric acid in real samples is achieved within 20 min. The leading, terminating and background electrolyte of the employed system consist of 10 mM HCl+beta-alanine+5 mM beta-cyclodextrin+0.05% hydroxy...

Lipid rafts represent a subcompartment of the plasma membrane that coordinate and regulate varieties of signaling processes while caveolins are the integral membrane protein of the lipid raft. To study the role of lipid raft in ischemic preconditioning (PC) of the heart, rat hearts were perfused by working mode and then preconditioned in absence or presence of a lipid raft disintegrator, Methyl...

The objectives of this study were to characterize a synthesis brom-isoflavanone (notated IF) with possible pharmacological activities, water insoluble and to examine the potential of beta-cyclodextrin to improve this property and obtain inclusion compounds that were analysed by different techniques: UV-Vis, IR, 1HNMR spectroscopy, thermal analysis. The presence of β-cyclodextrin rises the conte...

A method was developed for the enantioseparation of ofloxacin, a member of the fluoroquinolones, using an anionic cyclodextrin-derivative with or without combination with a neutral cyclodextrin-derivative, as the chiral selector (s) in an electrokinetic chromatography system. The best results were obtained with 0.35 mM sulfated beta-cyclodextrin dissolved in a 50 mM phosphate buffer, pH 2.5, an...