Enterobacteriaceae species are important human pathogens while increasing number of antibiotic resistant strains are detected worldwide. The most common antibiotic resistance in Enterobacteriaceae is observed against beta-lactams, fluoroquinolones, aminoglycosides while recently resistance to polymyxins has also appeared. Beta-lactam resistance is mainly conferred by beta-lactamases, capable to...

Escherichia coli isolates from various sources 2018 to 2019 were included in the study. Mcr-1 genes found two of 105 animal strains (2%) and seven 928 human (0.8%). All mcr-1-positive showed a low level resistance colistin (MIC ranged 4 8 µg/ml). Both isolated animals remained sensitive betalactam antibiotics did not contain beta-lactamase genes. Beta-lactamases absent only one humans. Four res...

BACKGROUND Ventilator-Associated Pneumonia (VAP) is the most frequent intensive-care-unit (ICU)-acquired infection. The aetiology of VAP varies with different patient populations and types of ICUs. METHODOLOGY A prospective study was performed over a period of 15 months in a tertiary care hospital to determine the various aetiological agents causing VAP and the prevalence of multidrug resista...

Beta-lactamase enzymes (BLs) have become the most worrisome mediators of antimicrobial resistance expressed by gram-negative bacteria (GNB). The multiple families of BLs have in common the ability to hydrolyze the -lactam rings of one or more members of the penicillins, cephalosporins, cephamycins, carbapenems, and monobactams. Extended-spectrum -lactamases (ESBLs) are a subset of BLs that conf...

Plasmid-encoded resistance to broad-spectrum cephalosporins and aztreonam is becoming a widespread phenomenon in clinical medicine. These antibiotics are inactivated by an array of different extended-spectrum beta-lactamases (ESBLs) which have evolved by point mutations of parental TEM or SHV beta-lactamases. In a previous study conducted during 1994-1995, SHV-2, SHV-2a and SHV-5 beta-lactamase...

Blood cultures drawn from a patient with clinically diagnosed invasive meningococcal disease, who had been previously administered benzylpenicillin, had beta-lactamases added to increase the probability of recovery of the causative organism. The blood cultures subsequently yielded Neisseria meningitidis but direct susceptibility tests by the comparative disk diffusion method demonstrated greatl...

The beta-lactamases of six Legionella species were characterized by isoelectric focusing, gel filtration, and substrate profiles. Fifteen strains of L. bozemanii, L. dumoffii, L. gormanii, L. longbeachae, and L. pneumophila produced beta-lactamases active against nitrocefin. L. micdadei enzymes previously reported to be beta-lactamase negative caused a very slow pH-dependent breakdown of nitroc...

background: pseudomonas aeruginosa is the causing agent of many hospital infections and metallo-beta-lactamases (mbl) are being reported with increasing frequency. the aim of this study was to determine the frequency of metallo-&beta-lactamases; (mbl) and vim-1 gene in multidrug-resistant strains of p. aeruginosa isolates and to compare the methods of phenotypic and molecular detection. materia...

At least 30 extended-spectrum beta-lactamases (ESB) have emerged responsible for resistance to indigestible beta-lactams (C3G) since their discovery in West Germany in 1983. Most of them are produced by enterobacteria and essentially K. pneumoniae which appeared susceptible to oxyimino-beta-lactams. A double-disk test was useful to detect such nosocomial isolates of enterobacteria (urines, bloo...