Cancer stem cells (CSCs) are a subset of cancer cells in tumors or established cancer cell lines that can initiate and sustain the growth of tumors in vivo. Cancer stem cells can be enriched in serum-free, suspended cultures that allow the formation of tumorspheres over several days to weeks. Brefeldin A (BFA) is a mycotoxin that induces endoplasmic reticulum (ER) stress in eukaryotic cells. We...

The purpose of this research is to develop and test a new approach prevent clostridial disease in cattle, based on the use compound biologically active feed additive (BFA). Some properties separate components BFA are characterized. showed that strain bacterium Bacillus amyloliquefaciens159 has an expressed antagonism toxin-producing strains C. perfringens. When using perfringens from ATCC colle...

Brefeldin A (BFA) rapidly blocks anterograde exocytotic transport through the Golgi complex. Sustained retrograde traffic induced by brefeldin A causes redistribution of constituents of the Golgi, but not the trans-Golgi network (TGN), to the endoplasmic reticulum (ER). In the present study on HepG2 cells, we have observed a differential effect of BFA on transport from the TGN of two soluble pr...

The in vivo effect of Brefeldin A (BFA) on the synthesis and transport of cell wall polysaccharides and proteins in the roots of pea seedlings (Pisum sativum L. cv. Alaska) was investigated. BFA (10 //gml") inhibited the synthesis of cell wall matrix polysaccharides by approximately 43%. Under the same conditions, cellulose synthesis was inhibited by approximately 77%. The percentage of incorpo...

This paper presents the implementation of the hybrid spiral-dynamic bacteria-chemotaxis (HSDBC) approach to control two different configurations of a two-wheeled vehicle. The HSDBC is a combination of bacterial chemotaxis used in bacterial forging algorithm (BFA) and the spiral-dynamic algorithm (SDA). BFA provides a good exploration strategy due to the chemotaxis approach. However, it endures ...

We have previously shown that glucocorticoids regulate the trafficking and processing of mouse mammary tumor virus (MMTV) proteins in viral-infected M1.54 rat hepatoma cells. To examine the role of intracellular membrane integrity on MMTV protein maturation, brefeldin A (BFA) was utilized to disrupt membrane flow between the endoplasmic reticulum and Golgi. Immunoprecipitation and immunofluores...

Brown adipocytes cultured from newborn combined-lipase-deficient (cld/cld) mice and castanospermine (CST)-treated 3T3-L1 adipocytes synthesize lipoprotein lipase (LPL) which is inactive and retained in the endoplasmic reticulum (ER) [Masuno, Blanchette-Mackie, Chernick and Scow (1990) J.Biol. Chem. 265, 1628-1638; Masuno, Blanchette-Mackie, Schultz, Spaeth, Scow and Okuda (1992) J. Lipid Res.33...

Binary Factor Analysis (BFA) is a typical problem of Independent Component Analysis (ICA) where the signal sources are binary. Parameter learning and model selection in BFA are computationally intractable because of the combinatorial complexity. This paper aims at an efficient approach to BFA. For parameter learning, an unconstrained binary quadratic programming (BQP) is reduced to a canonical ...

Binary Factor Analysis (BFA) uncovers the independent binary information sources from observations with wide applications. BFA learning hierarchically nests three levels of inverse problems, i.e., inference of binary code for each observation, parameter estimation and model selection. Under Bayesian YingYang (BYY) framework, the first level becomes an intractable Binary Quadratic Programming (B...

A brefeldin A (BFA)-inhibited guanine nucleotide-exchange protein (GEP) for ADP-ribosylation factors (ARF) was purified earlier from bovine brain cytosol. Cloning and expression of the cDNA confirmed that the recombinant protein (p200) is a BFA-sensitive ARF GEP. p200 contains a domain that is 50% identical in amino acid sequence to a region in yeast Sec7, termed the Sec7 domain. Sec7 domains h...