The quantity and localization of two lactose-binding lectins with molecular weights of 31,000 and 14,500 in human colorectal carcinoma tissue specimens obtained by surgical resection have been studied using specific polyclonal antibodies. Electrophoretic separation and blotting of detergent extracts of tumor tissues (48 specimens), followed by the binding of an antibody that recognizes both of ...

Titration calorimetry measurements of the binding of methyl alpha-D-mannopyranoside (Me alpha Man), D-mannopyranoside (Man), methyl alpha-D-glucopyranoside (Me alpha Glu), and D-glucopyranoside (Glu) to concanavalin A (Con A), pea lectin, and lentil lectin were performed at 281 and 292 K in 0.01 M dimethylglutaric acid-NaOH buffer (pH 6.9) containing 0.15 M NaCl and Mn+2 and Ca+2 ions. The site...

The lectin hepatic binding protein has a specific binding capacity for desialylated serum glycoproteins and is limited to hepatocyte membranes. This binding capacity was reduced by approximately 60% in the neoplastic nodules which resulted from exposure of rat livers to W-2-acetylaminofluorene. The binding capacity of the primary hepatocellular carcinomas which resulted from this regimen was re...

To characterize the interaction potential of the human vaginal isolate Lactobacillus plantarum CMPG5300, its genome was mined for genes encoding lectin-like proteins. cmpg5300.05_29 was identified as the gene encoding a putative mannose-binding lectin. Phenotypic analysis of a gene knock-out mutant of cmpg5300.05_29 showed that expression of this gene is important for auto-aggregation, adhesion...

Glycans or carbohydrates attached to therapeutic glycoproteins can directly affect product quality, safety and efficacy, and therefore must be adequately analyzed and controlled throughout product life cycles. However, the complexity of protein glycosylation poses a daunting analytical challenge. In this study, we evaluated the utility of a lectin microarray for assessing protein glycans. Using...

The human gut parasite Entamoeba histolytica, uses a lectin complex on its cell surface to bind to mucin and to ligands on the intestinal epithelia. Binding to mucin is necessary for colonisation and binding to intestinal epithelia for invasion, therefore blocking this binding may protect against amoebiasis. Acquired protective immunity raised against the lectin complex should create a selectio...

Helicobacter pylori is able to utilize several lectin-like, protein-carbohydrate interactions for binding to mucins, cell surfaces, and extracellular matrix proteins. As determined by hemagglutination assays and binding of radiolabeled bacteria to glycosphingolipids on thin-layer chromatograms, strains of gastric helicobacters and enterohepatic helicobacters, including Helicobacter canis, Helic...

Greg Parsonage, Emily Trebilcock, Marta A Toscano, Germán A Bianco, Juan M Ilarregui, Christopher D Buckley & Gabriel A Rabinovich† Hospital de Clínicas “José de San Martín”, Rabinovich, División Inmunogenética, Facultad de Medicina, Universidad de Buenos Aires, Av. Córdoba 2351, 3 Piso (C1120), Ciudad de Buenos Aires, Argentina Tel.: +54 115 950 8755; Fax: +54 115 950 8758; [email protected]

The lectin hepatic binding protein has a specific binding capacity for desialylated serum glycoproteins and is limited to hepatocyte membranes. This binding capacity was reduced by approximately 60% in the neoplastic nodules which resulted from exposure of rat livers to W-2-acetylaminofluorene. The binding capacity of the primary hepatocellular carcinomas which resulted from this regimen was re...