High affinity folate binding protein (HFBP) in porcine serum was purified 2,000-fold to a specific activity of 1.4 nmol of tetrahydrofolic acid (THF) bound per mg of protein, using folic acid (FA) coupled EAH-Sepharose gel affinity chromatography. Binding activity of purified HFBP to folate was examined by ultrafiltration method linked to high-performance liquid chromatography with electrochemi...

Influenza virus infects cells by binding to sialylated glycans on the cell surface. While the chemical structure of these glycans determines hemagglutinin-glycan binding affinity, bimolecular affinities are weak, so binding is avidity-dominated and driven by multivalent interactions. Here, we show that membrane spatial organization can control viral binding. Using single-virus fluorescence micr...

the characteristics of manganese and iron binding to human apotransferrin (apo-tf) have been investigated and compared in this study. both metal ions were taken up by human apo-tf and formed complexes, with the maximum absorbances observed at 410 and 340 nm for manganese-transferrin (mn-tf) and 465 nm for iron-transferrin (fe-tf). addition of manganese (1.5 µg/ml) to the reaction mixture contai...

Breeding companies want to use semen from bulls as soon possible take advantage of their desirable genetics. It takes several weeks for the sperm quality young stabilize and post-thaw become acceptable artificial insemination. Seminal plasma proteins protect spermatozoa during cryopreservation; it may some time seminal protein profile stabilize. The purpose this study was determine if can be us...

FET fusion proteins (FUS/EWS/TAF15) are critical in many human cancers, like sarcomas and leukemia. Interestingly, at the molecular level, they also form biomolecular condensates. However, underlying biophysical mechanism of these condensates on DNA remains poorly understood. Studies using single-molecule method, Curtains, demonstrated that finite sized droplets via liquid-liquid phase separati...

Photoelectron spectroscopy combined with the liquid microjet technique enables the direct probing of the electronic structure of aqueous solutions. We report measured and calculated lowest vertical electron binding energies of aqueous alkali cations and halide anions. In some cases, ejection from deeper electronic levels of the solute could be observed. Electron binding energies of a given aque...

TM-2 known as a potential antitumor drug is a novel semi-synthetic taxane derivative. As drug-protein interactions contribute to insights into pharmacokinetic and pharmacodynamic properties, we elucidated the binding of TM-2 to plasma protein. In this study, a simple, rapid and reliable method was developed and validated employing equilibrium dialysis for the separation of bound and unbound dru...

Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding was tested following repetitive washes or f...