Kakkalide is a major isoflavonoid from the flowers of Pueraria lobata (Willd.) Ohwi, possessing the protective effect against ethanol-induced intoxication and hepatic injury. The metabolism of kakkalide was investigated in rats. Thirteen metabolites were isolated by using solvent extraction and repeated chromatographic methods and identified by using spectroscopic methods including UV, IR, mass...

Biochanin A and formononetin are methylated isoflavones, abundant in legumes, which can be converted genistein daidzein by means of an O-demethylation reaction. Our objective was to transform these isoflavones into higher biological activity bioavailability compounds, such as genistein. Prior screening, using 10 lactic acid bacteria (LAB) 3 bifidobacteria strains, revealed that only 7 lactobaci...

This study determined the cytochrome P450 (P450) isoforms responsible for metabolism of isoflavones using human liver microsomes (HLM) and expressed P450s. The primary metabolite of genistein is 3'-OH-genistein, as identified with an authentic chemically synthesized standard. CYP1A2 was predominantly responsible for 3'-OH-genistein formation since its formation was inhibited (>50%, p < 0.05) by...