Phthalates are known as endocrine disruptors and common in plastic polymers, varnishes, printing inks. However, they most often enter the human body through food. Plastic materials that hold food contain different chemicals, phthalates one of them. can also be found microplastics since microplastic particles serve a vector for chemicals slowly released into beverages. The aim this preliminary s...

Objective(s) In recent years concerns have been raised regarding the incidence of male reproductive disorders from exposure to endocrine disruptors. So, chronic effects of di(2-ethylhexyl)phthalate were studied on histological and stereological structure of testis in adult Wistar rats. Materials and Methods Thirty two adult Wistar rats were randomly divided in four equal experiment groups; o...

During our recent work on the identification of drugs involved in overdose cases (1, 2), we have had occasion to investigate the constituents found in blood, urine, and gastric juices by combined GC-mass spectrometry. In a surprisingly large number of cases, we have observed, in addition to any drugs present, one or more peaks corresponding to phthalate esters (PAEs). Most commonly these were d...

The article provides information on the development of a new reference material (RM) for composition solution six priority phthalates (dimethyl phthalate, diethyl di(n-butyl) benzyl butyl bis(2-ethylhexyl) di(n-octyl) phthalate) in methanol provided with metrological traceability to GET 208-2019 (GET 208). procedure preparation and certification RMs is shown, including estimation homogeneity st...

BACKGROUND Phthalates are widely used as plasticizer and are considered as a typical endocrine-disrupting chemical. Epidemiological studies have associated serum or urinary phthalate metabolites with the prevalence of type 2 diabetes or related phenotypes. However, direct evidence supporting a causal role for exposure to phthalates in type 2 diabetes is lacking. METHODS To determine the poten...

Phthalates are plasticisers added to a wide variety of products, resulting in measurable exposure of humans. They are suspected to disrupt the thyroid axis as epidemiological studies suggest an influence on the peripheral thyroid hormone concentration. The mechanism is still unknown as only few in vitro studies within this area exist. The aim of the present study was to investigate the influenc...

Di-(2-ethylhexyl) phthalate, one of the phthalates most widely distributed in the environment, causes reproductive toxicity that is attributable to the action of its primary metabolite, mono-(2-ethylhexyl) phthalate (MEHP). Here, we have investigated the effects of MEHP on steroidogenesis by primary cultures of rat Leydig cell progenitors and immature granulosa cells. This phthalate stimulated ...

Environmental pollutants have recently emerged as potential risk factors for metabolic diseases, urging systematic investigation of pollutant effects on metabolic disease processes. To enable risk assessment of these so-called metabolic disruptors the use of stable, robust and well-defined cell based screening systems has recently been encouraged. Since beta-cell (dys)functionality is central i...

1 Identification of di (2-ethylhexyl) phthalate-induced carboxylesterase 1 in C57BL/6 mouse liver microsomes: Purification, cDNA cloning, and baculovirus-mediated expression.