A reproducible animal model of interstitial pulmonary fibrosis is generated in rodents by intratracheal in jection of the antineoplastic drug, b leomycin sulfate. Pulmonary lesions resemble closely those observed in human patients with fibrosis. Fibrot ic lesions are most pronounced 3 4 weeks after the drug's instil lation. 1 Histologically, increased interstitial connect ive tissue and hyperce...

The " DNA-membrane complex" (1200S-400S) obtained by lysis of rat ascites hepatoma cells in a low concentration of sodium dodecyl sarcosinate-sodium deoxycholate sedimented through neutral sucrose gradient more rapidly than the free DNA" (200S) which was released by a high concentration of sodium dodecyl sulfate. The former was decomposed by Pronase in the gradient but the latter was not affect...

In an attempt to develop better combination therapies for use with local radiation, the interaction between bleomycin and hyperthermia +/- radiation was studied in the FSaIIC tumor system. In cells exposed in vitro to bleomycin at 37 degrees C and at pH 7.40, the drug was substantially more toxic toward normally oxygenated than hypoxic cells. At hyperthermic temperatures (42 degrees or 43 degre...

The association of exposure to bleomycin with the development of scleroderma-like cutaneous abnormalities has been reported. We experienced a case of scleroderma involving the hands, feet, and forearms after bleomycin chemotherapy. The present report supports the possible causal relation of bleomycin with scleroderma. Regarding the widespread use of bleomycin, this complication is thought to be...

BACKGROUND Venous malformations (VMs) are common vascular anomalies; 40% are found in the head and neck. Percutaneous sclerotherapy is 1 treatment of choice. METHODS In all, 17 patients with facial VMs were treated by percutaneous sclerotherapy using alcohol and individually matched to lesions treated with bleomycin. Treatment details and outcomes were compared. The average numbers of session...

Cellular resistance to bleomycin is important in the treatment of neoplasms. However, the mechanism of bleomycin resistance has been not well investigated. The resistance may be attributed to alterations of membrane transport, binding to DNA or other cell components, an inactivating enzyme, and/or a DNA repair system. MIYAKI et al. have examined the binding of [11C]bleomycin to DNA in bleomycin...

A cDNA encoding human bleomycin hydrolase, a member of the cysteine proteinase family of proteins, has been cloned from a human brain cDNA library. The isolated cDNA contains an open reading frame coding for a polypeptide of 456 amino acids that contains all of the structural features characteristic of cysteine proteinases, including the cysteine, histidine, and asparagine residues that are ess...

The aerobic oxidation of Co(II)bleomycin bound to calf thymus DNA has been investigated in relation to the mechanism of reaction in solution in the absence of DNA. Kinetics of dioxygenation of the Co(II) complex were followed by spectrophotometric and electron spin resonance spectroscopy as well as dioxygen analysis. The reaction is slower than when carried out in solution; its rate is inversel...