Chemiluminescent western blotting has been in common practice for over three decades, but its use as a quantitative method for measuring the relative expression of the target proteins is still debatable. This is mainly due to the various steps, techniques, reagents, and detection methods that are used to obtain the associated data. In order to have confidence in densitometric data from western ...

Panel A in Fig 4 and panels A and F in S4 Fig are not presented correctly. The lanes in the corrected figures are separated by tracks for other mutants that are not relevant for the current work and can be seen in the original blots, provided here as S7 and S8 Figs. The authors apologise for the mistake and have provided corrected versions, along with the original blots that were used to create...

A general method for obtaining species-specific repetitive DNA sequences is described. The method is based on the detection of recombinant DNA clones containing repetitive sequences using labeled total genomic DNA. These repetitive DNA sequences can be used to identify individual mosquito adults, pupae, and larvae squashed on filter membranes (squash blots). This technique was used to distingui...

A polymerase chain reaction probe with 100% sequence identity to 120 deoxyribonucleotides of Fusobacterium nucleatum Fev1, coding for a part of the 40-kDa major outer membrane protein, was labeled with the steroid hapten digoxigenin. The probe was compared with various degenerate oligonucleotide probes and found to tolerate much more stringent washing conditions. It was therefore superior in di...

Moderately induced genes often escape detection in conventional subtraction hybridisation cloning. Here a modification of a phagemid subtraction protocol is described that overcomes this problem. The protocol uses low ratio hybridisation of driver to target sequences to allow enrichment of the sequences of interest, and back-hybridisation of the subtracted sequences with induced sequences to re...

The decay of signal over time was performed using serial dilution of a E. coli cell lysate containing a 51 kD polyhistidinetagged recombinant protein run on a 4–20% linear gradient Criterion gel. Blots were probed with mouse anti-polyhistidinetag primary antibody followed by incubation with GAM-HRP secondary antibody and StrepTactin HRP (1:10,000) for 1 hr each. The blots were then cut into two...

Due to the influence of peculiar material characteristics, austenitic stainless steel bolts are always easy be locked during disassembly. This is not only seriously affected service performance bolts, but also affects reuse bolts. In this paper, main causes and mechanism locking blots in process fastening summarized, effective measures containing correct selection, carefully clean before assemb...