Bovine primary uterine endometrial epithelial cells (EECs) are not ideal for long-term studies, because primary EECs lose hormone responsiveness quickly, and/or they tend to have a short life span. The aims of this study were to establish immortalized bovine EECs and to characterize these cells following long-term cultures. Immortalized bovine EECs were established by transfecting retroviral ve...

background: previous studies reported many discrepancies about the effects of corpus luteum (cl) and ovarian follicle size on the developmental competence of oocytes. objective: the aim of this study was to investigate the effects of cl and different size of follicle on the developmental potential of bovine oocytes. materials and methods: after ovarian classification based on presence or absenc...

introduction: the purpose of this study was to compare the inhibitory effect of bovine serum albumin (bsa) and hydroxyapatite (ha) on the antibacterial activity of white-colored mta (wmta) against staphylococcus (s.) aureus and streptococcus (s.) mutans after 24 and 72 hours . materials & methods: all materials were prepared according to the manufacturer’s directions immediately before testing....

background: rotaviruses (rv) are important viral diarrheal agents in calves. vaccination is an optimum measure to prevent bovine rotaviruses (brv) infection. however, little research on brv vp7 vaccine has been done and currently there is no brv vaccine. objective: to prepare a subunit vaccine of brv and investigate its efficacy. methods: total rna was extracted from ma104 cells infected with b...

in most countries, tuberculosis caused by mycobacterium bovis is mainly a disease of cattle but can infect buffalos too. the disease can be controlled successfully by mean of a test-and-slaughter program. in iran test-and-slaughter program has started since 1971 and prevalence of bovine tuberculosis reduces from 5% to less than 0.12% in recent years. in western azarbaijan, north west of iran, t...

to investigate uterine and serum ca++ and mg++ variations during the estrous cycle in the bovine, 66 genital tracts and blood samples were collected from urmia abattoir, urmia, iran. the phase of the estrous cycle was determined by examination of the structures present on ovaries and uterine tonicity. of the collected samples, 17 were pro-estrus, 12 estrus, 14 metestrus and 23 diestrus. the ute...

a rapid and sensitive reverse transcription polymerase chain reaction (rt-pcr) and nested-pcr were used to detect bovine viral diarrhea virus 1 (bvdv-1) in bull semen. selected primers could amplify a part of the 5´utr of the bvdv genome. a 294 bp dna fragment was amplified and specificity of the results was confirmed by direct sequencing of the pcr product. prior to rna extraction, the seminal...

The effects of the human and bovine LSF (derived from sera) as well as their purified fractions were studied on murine lymphocytes reactions indicated by blast transformation (BT) assay, mixed lymphocyte culture (MLC) and IgG synthesis. The results indicated that bovine lipid suppressor factor (LSF) has significant immunosuppressive activity on lymphocytes proliferation both in BT and MLC ...