The hydroaromatic acids shikimate and quinate, which may be available as carbon sources in the soil, supported production of only low levels of acidic exopolysaccharide by Bradyrhizobium japonicum. Exopolysaccharide production (micrograms per 10 cells) was 4.9 on quinate and 4.5 on shikimate; in comparison, it was 128 on adipate, 18 on l-arabinose, and 39 on d-glucose.

Free-living soybean rhizobia and Bradyrhizobium spp. (lupine) have the ability to catabolize ethanol. Of the 30 strains of rhizobia examined, only the fast- and slow-growing soybean rhizobia and the slow-growing Bradyrhizobium sp. (lupine) were capable of using ethanol as a sole source of carbon and energy for growth. Two strains from each of the other Rhizobium species examined (R. meliloti, R...

Enzyme electrophoresis and rRNA sequencing indicated that root nodule bacteria from the canopy tree Platypodium elegans and the lianas Machaerium milleflorum and Machaerium arboreum on Barro Colorado Island, Panama, were highly diverse on a local scale. A total of 11 distinct multilocus genotypes [ETs (electrophoretic types)] was found among the 33 isolates analysed. On average, ETs differed fr...

Bradyrhizobium japonicum strain FN1 was found to produce bacteriocin-like zones of clearing when tested against other strains of bradyrhizbia. The genome was sequenced, and several putative bacteriocin-producing genes, in addition to the expected genes involved in nodulation and nitrogen fixation, were identified.

El objetivo del presente trabajo fue evaluar el efecto de bioproductos en crecimiento, producción y estado fitosanitario plantas nabo, teniendo cuenta la viabilidad uso quitosano e inoculantes elaborados a partir cepas Bradyrhizobium japonicum, para contribuir con prácticas más sostenible este cultivo. La investigación realizada campus “La María” Universidad Técnica Estatal Quevedo, provincia l...

La presente investigación se desarrolló para determinar la respuesta agronómica de plantas tomate (Solanum lycopersicum L.) a aplicación Bradyrhizobium japonicum y quitosano alto bajo peso molecular. Para el análisis, establecieron 6 tratamientos 1) Quitosano molecular, 2) molecular; 3) B. japonicum; 4) + 5) molecular 6) testigo experimental con 3 repeticiones distribuidos aleatoriamente en un ...

Legumes interact with nodulating bacteria that convert atmospheric nitrogen into ammonia for plant use. This nitrogen fixation takes place within root nodules that form after infection of root hairs by compatible rhizobia. Using cDNA microarrays, we monitored gene expression in soybean (Glycine max) inoculated with the nodulating bacterium Bradyrhizobium japonicum 4, 8, and 16 days after inocul...

Forty-nine isolates of Bradyrhizobium japonicum indigenous to a field where soybeans were grown for 45 years without inoculation were characterized by using four DNA hybridization probes from B. japonicum. nifDK-specific hybridization clearly divided the isolates into two divergent groups. Diversity in repeated-sequence (RS)-specific hybridization was observed; 44 isolates derived from 41 nodul...

Bradyrhizobium japonicum possesses three soluble c-type cytochromes, c550, c552, and c555. The genes for cytochromes c552 (cycB) and c555 (cycC) were characterized previously. Here we report the cloning, sequencing, and mutational analysis of the cytochrome c550 gene (cycA). A B. japonicum mutant with an insertion in cycA failed to synthesize a 12-kDa c-type cytochrome. This protein was detecta...