BACKGROUND Previously published equations to adjust calcium for albumin concentration may vary depending on factors such as the type of reagents used. Albumin-adjusted calcium equations derived from laboratories using the bromocresol purple (BCP) albumin binding reagent have not been described. METHODS The linear regression equation for the binding of calcium and BCP-albumin was derived in a ...

Among Streptococcus thermophilus cultures, the principle component of yoghurt and cheese starters, a minority of strains forms the group of 'H'-strains which show an unusually high acidification rate, grow faster and coagulate milk 3-5 hours earlier than the typical S. thermophilus cultures. A large-scale screening study was performed to select 'H'-strains of S. thermophilus from more than 100 ...

The mitochondrial carriers are a family of transport proteins that, with a few exceptions, are found in the inner membranes of mitochondria. They shuttle metabolites and cofactors through this membrane, and connect cytoplasmic functions with others in the matrix. SAM (S-adenosylmethionine) has to be transported into the mitochondria where it is converted into S-adenosylhomocysteine in methylati...

Pyrimidine (deoxy)nucleoside triphosphates are required in mitochondria for the synthesis of DNA and the various types of RNA present in these organelles. In Saccharomyces cerevisiae, these nucleotides are synthesized outside the mitochondrial matrix and must therefore be transported across the permeability barrier of the mitochondrial inner membrane. However, no protein has ever been found to ...

bacteriorhodopsin operates as a light/proton transfer pump which converts the light energy into a proton gradient. energy stored in the proton gradient can be used in a variety of ways. bacteriorhodopsin producing sources are some halobacterium species such as halobacterium sodomense and halobacterium salinarum which continue to grow in harsh and salt-saturated conditions. two types of red pigm...

A sensitive spectrophotometric method for the determination of dopamine was carried out without any separation steps. Bromocresol green is adsorbed on Sephadex LH-20 gel but the sorption decreases in the presence of dopamine due to ion-pair formation between bromocresol green and dopamine in solution. This attenuation was used to the microdetermination of dopamine by measurement of absorbance o...

Chromosphere such as triaryl methane family of dyes used in various industries can affect the body living organism system upon interaction with most biochemical molecule is DNA. In this research, binding mode chromophore that Bromothymol blue, Bromophenol Bromocresol green and Malachite double strand DNA phosphate buffer at pH 6.9 was investigated through UV/VIS spectroscopy. The study shows bl...