The effects o f thidiazuron (TDZ) on adventitious shoot bud formation from leaf and peti­ ole explants excised from in-vitro-grown plantlets o f a begonia were tested by a two-step procedure: a short exposure (5 to 120 min) to 40 pM TDZ in liquid MS medium followed by culture on agar-solidified MS medium without growth regulators. Exposure of explains for five min to TDZ induced bud formation, ...

Differentiation of metanephric mesenchyme is triggered by an inductive signal(s) from the epithelial ureteric bud. As a result of this induction, most of the metanephric mesenchyme converts into epithelium of a nephron. We have developed and characterized an explant culture system, in which metanephric mesenchyme can grow and completely differentiate in vitro in the absence of an inductive tiss...

Adult stem cells provide replacement and repair descendants for normal turnover or injured tissues. These cells have been isolated and expanded in culture, and their use for therapeutic strategies requires technologies not yet perfected. In the 1970s, the embryonic chick limb bud mesenchymal cell culture system provided data on the differentiation of cartilage, bone, and muscle. In the 1980s, w...

in this study, the effects of different types of hand plucking were studied on green leaf yield and black tea quality. bud and a leaf, bud and two leaves, bud and three leaves and bud and four leaves were the types of hand plucking. plucking was down on chine’s hybrid tea and clone 100 in fuman tea research station. shoots were plucked in flashing period regularly during three years. green leaf...

abstract persian walnut is one of the most important nut crops in iran. now the majority of orchards are seedling with a great diversity. for establishing uniform orchards and increasing the quantity and quality of yield, patch budding as the most suitable propagation methods, have still technical and practical problems in the country. in spite of high percent of primary success in patch buddin...

Factors affecting reliable plant regeneration from unfertilized ovule culture of gentians (Gentiana spp.) were examined. Cold pretreatment (4°C) of flower buds enhanced or maintained production of embryo-like structure (ELS). When 43 genotypes were surveyed in two different labs, 40 of them produced ELSs ranging from 0.01 to 26.5 ELSs per flower bud. No ELSs could be obtained in three genotypes...

in order to study, in vitro bud induction and shoot regeneration of different explants (shoot tip, axillary bud, hypocotyl and cotyledon) of henbane on ms medium enriched with various concentrations of kinetin (0, 1, 3 and 5 mg.l-1) alone or in combination with indole-3 acetic acid (0, 0.1 and 0.5 mg.l-1) this research was investigated in urom zist tak knowledge-based company plant tissue cultu...

1. Introduction Since Solursh and his coworkers set up the limb bud micromass cultures in 1977 (1), this procedure has become a major model to analyze cellular and molecular event involved in chondrogenesis (2–7). Recently, RCAS retroviral vectors have been developed that can infect chicken embryos in ovo. These have advanced our understanding in limb bud patterning remarkably (8). Here we desc...

Elongation of the mouse anteroposterior axis depends on a small population of progenitors initially located in the primitive streak and later in the tail bud. Gene expression and lineage tracing have shown that there are many features common to these progenitor tissues throughout axial elongation. However, the identity and location of the progenitors is unclear. We show by lineage tracing that ...