weight), cultivated anaerobically at 37°C for 24hr in a medium containing 0.4% casamino acids, 0.3% yeast extract, 0.3% peptone, 1.0% glucose, 0.15% sodium chloride, 0.1% Tween 80 and 0.04% L-cysteine, pH 7.2, were disrupted by passing through a French pressure cell (Ohtake Works Co.) at 1500-1700kg/cm2. Nucleic acids were removed from the cell extracts by 1%streptomycin treatment, and then the...

This paper presents a new approach to global clock distribution in which traditional tree-driven grids are augmented with onchip inductors to resonate the clock capacitance at the fundamental frequency of the clock node. Rather than being dissipated as heat, the energy of the fundamental resonates between electric and magnetic forms. The clock drivers must only provide the energy necessary to o...

This paper describes a method to reconstitute GPCRs into an array of giant liposomes in a buffer solution. We first achieved the formation of giant liposomes in a buffer solution by adding fructose to a lipid film; normally such giant liposomes are formed by using only pure waters (MilliQ). Moreover, we successfully reconstituted adrenergic receptors (AR) of GPCRs into the giant liposome array ...

In the reordering buffer problem (RBP), a server is asked to process a sequence of requests lying in a metric space. To process a request the server must move to the corresponding point in the metric. The requests can be processed slightly out of order; in particular, the server has a buffer of capacity k which can store up to k requests as it reads in the sequence. The goal is to reorder the r...

Instruction-set randomization (ISR) obfuscates the “language” understood by a system to protect against code-injection attacks by presenting an ever-changing target. ISR was originally motivated by code injection through buffer overflow vulnerabilities. However, Stuxnet demonstrated that attackers can exploit other vectors to place malicious binaries into a victim’s filesystem and successfully ...

Buffer overflow attacks are among the oldest and most common attacks used to gain privileged access to a computer system. In fact, statistics from Carnegie Mellon’s Computer Emergency Response Team reveal that buffer overflow vulnerabilities accounted for about 50% of all reported vulnerabilities in 2000 [2]. It is surprising that after almost 20 years of buffer overflow exploits, they are stil...

Polymorphism. TaqI restriction fragment length polymorphism (RFLP) in the stearoyl-coenzyme A terminal desaturase ( SCDl) gene of purebred Japanese Black cattle. Source and Description of Probe. A 2.7-kb cDNA insert was excised from a pSp86 clone by digestion with EcoRI and ScaI. The insert contains the complete coding sequence of the rat SCDl gene (Thiede et al., 1986). Method of Detection. Pr...

Here we demonstrate the use of multiple Si nanochannel (NC) or nanograting (NG) instead of the conventional single nanochannel or nanowire design in biosensors. The NG devices can significantly reduce device-to-device variation, and improve device performance, e.g. higher current, higher ON/OFF ratio, smaller subthreshold slope, lower threshold voltage Vt in buffer solution. NG devices also res...