We examined the effects of the activation of metabotropic P2Y receptors on the intracellular Ca(2+) concentration and the release of neuropeptide calcitonin gene-related peptide (CGRP) in isolated adult rat dorsal root ganglion neurons. In small-sized dorsal root ganglion neurons (soma diameter<30 microm) loaded with fura-2, a bath application of ATP (100 microM) evoked an increase in intracell...

background: the present study investigated the effect of the middle east dust storm episodes on the concentration and composition of pm 10 during april to september 2013 in sanandaj city, western iran. methods: sampling was once every six days, and on dusty days using an omni air sampler. the pm 10 sample was collected on polytetrafluoroethylene filters. average of 24 h values of pm 10 mass con...

Stomatal density of pods and leaves were determined for six commercial snap bean cultivars (Phaseolus vulgaris L. ‘Evergreen’, ‘Hystyle’, Labrador’, ‘Tenderlake’, ‘Top Crop’, and ‘Venture’) grown at three planting dates, in an attempt to find morphological traits that could be related to cultivar differences in pod Ca concentration. Snap beans were planted three times at ≈1-week intervals begin...

Transient transfection of Chinese hamster ovary or baby hamster kidney cells expressing the Group I metabotropic glutamate receptor mGlu1alpha with green fluorescent protein-tagged pleckstrin homology domain of phospholipase Cdelta1 allows real-time detection of inositol 1,4,5-trisphosphate. Loading with Fura-2 enables simultaneous measurement of intracellular Ca(2+) within the same cell. Using...

The multiplicity of mechanisms involved in regulation of intracellular Ca(2+) concentration ([Ca(2+)](i)) in smooth muscle results in both intra- and intercellular heterogeneities in [Ca(2+)](i). Heterogeneity in [Ca(2+)](i) regulation is reflected by the presence of spontaneous, localized [Ca(2+)](i) transients (Ca(2+) sparks) representing Ca(2+) release through ryanodine receptor (RyR) channe...

in order to investigate the effect of mycorrhizal fungi and phosphorus fertilizer on concentration of leaf nutrients and photosynthetic pigments of bean (phaseolus vulgaris l.) in condition of irrigation with saline water, an experiment was conducted based on completely randomized design with three replications in greenhouse of bahonar university of kerman, iran during 2010. the studied factors...

Neurons are mechanically robust. During prolonged swelling, molluscan neurons can triple their apparent membrane area. They gain surface area and capacitance independent of extracellular Ca concentration ([Ca]e), but it is unknown if an increase in intracellular Ca concentration ([Ca]i) is necessary. If Ca for stimulating exocytosis is unnecessary, it is possible that swelling-induced membrane ...

This study explored the effect of deltamethrin, a pesticide, on intracellular free Ca²⁺ concentration ([Ca²⁺]i) in PC3 human prostate cancer cells. Deltamethrin at concentrations between 5 μM and 20 μM evoked [Ca²⁺]i rises in a concentration-dependent manner. This Ca²⁺ signal was inhibited by 22% by removal of extracellular Ca²⁺. Nifedipine, econazole, and SKF96365 also inhibited the Ca²⁺ signa...

Mitochondria play an important role in intracellular Ca(2+) concentration ([Ca(2+)]i) regulation in the heart. We studied sarcoplasmic reticulum (SR) Ca(2+) release in cat atrial myocytes during depolarization of mitochondrial membrane potential (ΔΨm) induced by the protonophore FCCP. FCCP caused an initial decrease of action potential-induced Ca(2+) transient amplitude and frequency of spontan...