Despite the enormous therapeutic potential of siRNA as a treatment strategy, the delivery is still a problem due to unfavorable biodistribution profiles and poor intracellular bioavailability. Calcium phosphate (CaP) co-precipitate has been used for nearly 40 years for in vitro transfection due to its non-toxic nature and simplicity of preparation. The surface charge of CaP will be tuned into p...

Maryam Nabiyouni, Sarit B Bhaduri. University of Toledo, Toledo, OH 43606 Statement of Purpose: Nonviral gene delivery has gained much attention due to its potential in addressing the viral gene delivery limitations like unintentional mutagenesis. Different natural and synthetic materials such as polymers, lipids, and calcium phosphate particles have been developed as DNA carriers. However, the...

This study aimed at preparing and studying the properties of nanoparticles of calcium phosphate (nCaP) with Ca/P ratios ranging from 1.0 to 1.67 using a spray-drying technique. Micro-structural analyses suggested that the nCaPs with Ca/P ratios of 1.67 to 1.33 were nano-sized amorphous calcium phosphate (ACP) containing varying amounts of acid phosphate and carbonate. The nCaP with Ca/P ratio o...

We report the synthesis of hydroxyapatite nanoparticles (HANPs) by the coprecipitation method using calcium D-gluconate and potassium hydrogen phosphate as the sources of calcium and phosphate ions, respectively, and the triblock copolymer F127 as a stabilizer. The HANPs were characterized using scanning electron microscopy, x-ray diffraction, and nitrogen adsorption/desorption isotherms. Remov...

Calcium phosphate nanoparticles (CPNPs) encapsulating small organic molecules, such as imaging agents and drugs, are considered to be ideal devices for cancer diagnosis or therapy. However, it is generally difficult to encapsulate small organic molecules in CPNPs because of the lack of solubility in water or binding affinity to calcium phosphate. To solve these issues, we utilized the carboxyme...

In recent years, nanoparticle systems have been frequently used as non-viral carriers for the treatment of genetically based diseases and cancer. However, conjugate formation should be optimized by selecting a suitable carrier transport each genetic material desired to transported target cell. A created taking into account size zeta potential electrical characteristics cargo being transported. ...