The aim of the present study was to analyze the mechanism by which nerve growth factor (NGF) promotes callus formation in mice with tibial fracture. NGF transgenic homozygotic mice and NGF wild homozygotic mice were selected to construct non-stabilized fracture model of tibia. The mice were sacrificed on days 7, 14 and 21, respectively, and each group had a sample with 8 mice at each point in t...

Tomato (Lycopersiconesculentum Mill. cv. Rampai) is rich in lycopene, a carotenoid group compound with many biological activities, one of which as an antioxidant. Conventional tomato cultivation not optimal due to the susceptibility plants pests and diseases, even though need for lycopene continues increase. Plant tissue culture method can be solution production. Callus Rampai was initiated fro...

Callus cultures from root explants of Lathyrus sativus L. ‘Derek’ were tested for their morphogenic capacity. Primary explants (fragments of roots) were cultivated on three induction media. We obtained three lines of callus tissue among which we identified two non-embryogenic lines and one embryogenic line. Callus originally cultivated on modified MS medium supplemented with 0.05 mgL picloram, ...

An in vitro culture system leading to the formation of callus and plant regeneration, starting from nodal sections and shoot tips, was developed for Solidago chilensis (Asteraceae). The content of the gastroprotective diterpene solidagenone as well as the phenolics chlorogenic acid (CA) and rutin was determined either in rhizomes from wild growing plants and in callus and in in vitro regenerate...

Feasible and efficient tissue culture plays an important role in plant genetic engineering. Wheat (Triticum aestivum L.) immature embryos (IMEs) are preferred for tissue culture to mature embryos (MEs) because IMEs easily generate embryogenic callus, producing large number of plants. The molecular mechanisms of regulation and the biological pathways involved in embryogenic callus formation in w...

The Silybum marianum (L.) Gaertn. is the dicotyledonous herbs of the Asteraceae family that is important in medical industry. The biological active compound of S. marianum is a mixture of several flavonolignals generally known as silymarin. The purpose of this study was to optimize S. marianum tissue culture for callus induction, silymarin production and comparison of some biochemical traits be...

background and objectives: grape has high nutritional value and it is an economically important horticultural plant in the world. iran has seventh producer grape rank in the world. today tissue culture is one of technique which can be used in grape propagation and breeding. material and method: in order to optimization of callus induction and micropropagation of grape in vitro condition, this i...

The present study was carried out to build up an efficient protocol for various concentrations of 2, 4D for “Callus formation and somatic embryogenesis in sugarcane (Saccharum spp L.) using various concentrations of 2, 4-D and RAPD analysis of regenerated plants in the laboratory of Biotechnology Nuclear Institute of Agriculture, Tandojam during the year 2015 to 2016. Three sugarcane varieties ...

We describe mutant tissue lines of Arabidopsis that are able to grow in vitro as callus on hormone-free medium. The 14 lines presented here show different hormone autotrophic differentiation behaviors that can be classified into three categories: (a) forming roots (rooty callus), (b) forming shoots or shoot-like structures (shooty callus), or (c) growing without organ formation (callus). Three ...

BACKGROUND AND AIMS Understanding the fate and dynamics of cells during callus formation is essential to understanding totipotency and the mechanisms of somatic embryogenesis. Here, the fate of leaf explant cells during the development of embryogenic callus was investigated in the model legume Medicago truncatula. METHODS Callus development was examined from cultured leaf explants of the high...