Barley is affected by Fusarium head blight. The mycotoxins produced by F. graminearum affect the grain safety, malting and beer quality. Barley can be irradiated with electron beam radiation to reduce the fungal contamination, but there may be some Fusarium strains which can survive, grow and produce mycotoxins as irradiated barley is malted. Random amplified polymorphic DNA (RAPD) analysis was...

Agmatine and other putrescines are known for being strong inducers of deoxynivalenol (DON) production in Fusarium graminearum. Other important species produce DON and/or other trichothecene type B toxins (3 acetylated DON, 15 acetylated DON, Fusarenon-X, Nivalenol), such as F. culmorum and F. poae. In order to verify whether the mechanism of the regulation of trichothecene type B induction by a...

Deoxynivalenol (DON), a mycotoxin produced by some Fusarium species, is a frequent contaminant of cereal. In the present study, 24 weanling piglets received either control feed or feed naturally contaminated with DON (2.8 mg/kg) for four weeks. Consumption of contaminated feed significantly reduced the animal weight gain during the first week of the experiment, but had a moderate effect on cult...

Modifications to a published method are described for the determination of deoxynivalenol (DON) in wheat by gas chromatography with electron capture quantitation of the heptafluorobutyrate derivative. In the modified method, DON is extracted by shaking the sample with methanol-water on a wrist-action shaker, followed by filtration through rapid flow paper. One concentration step is eliminated, ...

Previous research showed that nursery pigs fed deoxynivalenol (DON) contaminated diets supplemented with spray-dried porcine plasma (SDPP) had similar growth rate and feed intake as those consuming noncontaminated diets. In this study, two blocks of 100 weanling pigs were used to determine the optimal inclusion level of spray-dried bovine plasma(SDBP) required in nursery diets contaminated with...

A total of 122 wheat varieties obtained from the Nordic Genetic Resource Center were infected artificially with an aggressive Fusariumasiaticum strain in a field experiment. We calculated the severity of Fusarium head blight (FHB) and determined the deoxynivalenol (DON) content of wheat grain, straw and glumes. We found DON contamination levels to be highest in the glumes, intermediate in the s...

Mycotoxins are secondary metabolites of fungi that grow on a variety of substrates. Due to their high consumption of cereals and their sensitivity, pigs are highly impacted by the presence of mycotoxins. At the European level, regulations and recommendations exist for several mycotoxins in pig feed. Among these toxins, fumonisin B1 (FB1), and deoxynivalenol (DON) have a great impact on the inte...

Contamination of crops used for food and feed production with Fusarium mycotoxins, such as deoxynivalenol (DON), raise important health and economic issues all along the food chain. Acute exposure to high DON concentrations can alter the intestinal barrier, while chronic exposure to lower doses may exert more subtle effects on signal transduction pathways, leading to disturbances in cellular ho...

Deoxynivalenol (DON) is a type B trichothecene mycotoxin that is commonly detected in cereals and grains world-wide. The low-tolerated levels of this mycotoxin, especially in mono-gastric animals, reflect its bio-potency. The toxicity of DON is conventionally attributed to its ability to inhibit ribosomal protein biosynthesis, but recent advances in molecular tools have elucidated novel mechani...

In this work, we studied the impact of harvesting time on Fusarium mycotoxin occurrence in spring wheat and effect contamination quality these grains. The grains (Triticum aestivum L.) were collected 2016–2018 when crop had reached full maturity, 10 ± 2 days 17 3 after maturity. grain samples analyzed for infection co-contamination with mycotoxins deoxynivalenol (DON), zearalenone (ZEA), T-2 to...