Roller pumps are widely used for cardiopulmonary bypass in developing nations by virtue of proven safety during several years of institutional use and cost effectiveness. However, careful adjustment of roller occlusion is needed because they are known to cause spallation, tubing wear, and the occasional incident of rupture of tubing in the extracorporeal circuit. Rupture of polyvinylchloride tu...

A failure analysis was performed on Norton S-65-HL Tygon® tubing. Fatigue testing was performed on four sizes of this tubing, and essentially showed how the tubing wears out. A dynamic "life hours to failure" test, which was performed on the 3/8" internal diameter (ID) size, quantified when the tubing ruptured. Based on results of laboratory testing and the institution's clinical extracorporeal...

A recently completed study quantified the percent of fentanyl or morphine sulfate lost to uncoated polyvinylchloride (PVC) tubing or to one of two hollow fiber oxygenators within the extracorporeal life support (ECLS) circuit. The results demonstrated the majority of drug loss was due to adsorption by the PVC tubing. The purpose of this study was to determine if a tubing coating process affects...

Tubing circuits primed with water were constructed on two roller pumps to characterize and compare two static methods of setting the occlusion on roller pumps (fluid drop rate and pressure drop rate). Twelve separate experiments were performed in which PVC boot tubing diameters (1/2 in, 3/8 in and 1/4 in), roller position (each roller alone on a two roller pump) and roller occlusion (total occl...

Optical absorption spectra of 0.2-mm filtered seawater samples originating from diverse oceanic and coastal waters were measured with a long pathlength capillary waveguide; results were compared with those of three different laboratory spectrophotometers. The 0.5-m-long 550-mm (inside diameter) aqueous waveguide uses only 120 ml of filtered seawater, making it convenient for use in flow-through...

BACKGROUND Electrophoresis on polymeric rather than glass microstructures is a promising separation method for analytical chemistry. Assays on such devices need to be explored to allow assessment of their utility for the clinical laboratory. METHODS We compared capillary and plastic microchip electrophoresis for clinical post-PCR analysis of hepatitis C virus (HCV). For capillary electrophore...

Khanina, Natalya. M.S., Purdue University, December 2014. Reverse-Phased Liquid Chromatography of Small Molecules using Silica Colloidal Crystals. Major Professor: Mary J. Wirth. Capillaries packed with a silica colloidal crystal (SCC) of 470 nm nonporous particles have recently been shown to offer significant improvements in the peak width, resolution, and speed of protein analysis due to the ...