The effect of Maillard reaction on red blood cells (RBC) deformability was investigated. Exposure of RBC to carbonyl compounds (dl-glyceraldehyde, glyoxal, glycolaldehyde, 3-deoxyglucosone, and d-glucose) leading to Maillard reaction caused a marked decrease in RBC deformability even at 1mM level. The decrease rate depended on the kind of carbonyl compounds, in which both dl-glyceraldehyde and ...

Many carbonyl species from either lipid peroxidation or glycoxidation are extremely reactive and can disrupt the function of proteins and enzymes. 4-hydroxynonenal and methylglyoxal are the most abundant and toxic lipid-derived reactive carbonyl species. The presence of these toxics leads to carbonyl stress and cause a significant amount of macromolecular damages in several diseases. Much evide...

In enzymology, as in organic chemistry, many transformations revolve around the chemistry of the carbonyl group. A carbonyl group is electrophilic how can it be made more so? Hydrogens next to a carbonyl group have a reduced pK, how can this reactivity be promoted? Nature has found several ways to address these mechanistic challenges, the most important of which is the use of imines. Studies on...

An n = π* interaction between neighboring carbonyl groups has been postulated to stabilize protein structures. Such an interaction would affect the (13)C chemical shielding of the carbonyl groups, whose paramagnetic component is dominated by n = π* and π = π* excitations. Model compound calculations indicate that both the interaction energetics and the chemical shielding of the carbonyl group a...

The difficult to handle and store zinc borohydride, Zn(BH4)2, is stabilized by supporting it on polyvinylpyridine. This new mild, efficient, selective, and regenerable polymer supported borohydride reducing agent reduces a variety organic compounds such as, aldehydes, a-b-unsaturated carbonyl compounds, acid chlorides, epoxides, disu...

the oxidation of lipids change organoleptical properties, nutritional value and shelf life of oils, and produce undesirable compounds that are not safe for customer”s health. there is many methods for prevention of oxidation such synthetic antioxidants, (bha, bht, tbhq, gallatester).synthetic antioxidants, have undesirable effects such cancer, motation in human body, therefore it was removed fr...

The extraction and quantitation of carbonyl metabolites from cell lysate was accomplished using a carbonyl-reactive fluorous tag and capillary liquid chromatography coupled to mass spectrometry (capLC-MS). Selective fluorous tagging for ketones and aldehydes provided a 30-fold increase in sensitivity using electrospray ionization MS. Separation of fluorous tagged carbonyl resulted in good separ...